γ射线辐照对春兰根状茎生长及抗氧化酶活性的影响  

EFFECT OF ^(60)Co γ-rays IRRADIATION ON GROWTH AND ANTIOXIDANT ENZYME ACTIVITIES OF RHIZOMES IN Cymbidium goeringii

作　　者：刘璐璐[1] 柴明良[1] 徐晓薇[2] 李方[1] 

机构地区：[1]浙江大学园艺系,浙江杭州310029 [2]浙江省亚热带作物研究所,浙江温州325005

出　　处：《核农学报》2008年第1期23-27,共5页Journal of Nuclear Agricultural Sciences

摘　　要：将春兰(Cymbidium goeringii)的离体根状茎切成长各约1cm的顶段、中段和末段,用60Coγ射线进行辐照,剂量分别为0、5、10、20、30和50Gy。测定了不同辐照剂量下新生根状茎的生长,研究了辐照原初根状茎愈创木酚过氧化物酶(G-POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)等抗氧化酶活性以及过氧化物酶同工酶谱的影响。结果表明:辐照剂量为30和50 Gy处理的根状茎虽能存活,但未有新生根状茎的发生,生长停滞。辐照原初根状茎上CAT和SOD的活性随着辐照剂量的增加均有先升高后降低的趋势,而G-POD的活性则表现为先略降低后升高。综合新生根状茎的生长、抗氧化酶活性及过氧化物同工酶谱的结果,春兰诱变育种的适宜剂量应在20~30Gy间,且最合适的诱变部位是根状茎的顶段。The rhizomes of Cymbidium goeringii were cut into tip, middle and basal segments with a length of about 1 cm, and the segments were irradiated with ^60Co 7-rays at doses of 0 ,5 , 10 ,20 ,30 and 50 Gy, respectively. The growth characters, the activities of antioxidant enzymes, including superoxide dismutase （ SOD）, catalase （CAT） and gnaiacol peroxidase （ G- POD）, and peroxidase isozyme of the were measured. The results showed that the segments irradiated at the dose of 30 and 50 Gy could survive, but couldn＇t form new rhizomes and the growth was almost stagnated. With the increase of the doses, the SOD and CAT activities of the rhizome were increased first, then significantly decreased, but the reverse effect was observed in G-POD activities, which was decreased first, then increased. Combining both the effect results on the growth characters, on activity changes of peroxidase isozyme and the changes of the anti-oxidant enzymes after irradiation, it was concluded that the appropriate doses of ^60Co 7-rays irradiation for mutation breeding of Cymbidium goeringii should be in the rage of 20 to 30 Gy, and the optimal part of the rhizome seems to be the tip segment.

关 键 词：春兰 辐射诱变 抗氧化酶 同工酶 

分 类 号：S682.31[农业科学—观赏园艺]