小麦微粒体对丙酯草醚的代谢作用初探  

PRELIMINARY STUDY ON METABOLISM OF PYRIBAMBENZ-PROPYL BY WHEAT MICROSOMES

作　　者：张泉[1] 黄建中[1] 杨征敏[2] 朱其松[1] 叶庆富[1] 吕龙[2] 徐步进[1] 陈子元[1] 

机构地区：[1]浙江大学原子核农业科学研究所／农业部、浙江省核农学重点实验室,浙江杭州310029 [2]中国科学院上海有机化学研究所,上海200032

出　　处：《核农学报》2008年第1期84-87,83,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金面上项目(10575086);国家自然科学基金重点项目(20632070);中国科学院知识创新工程重要方向项目(KGCX3-SYW-203-03);浙江省科技厅院士基金

摘　　要：用5%乙醇和10mmol/L丙酯草醚诱导的小麦黄化苗茎部制备微粒体,与[C环-U1-4C]丙酯草醚保温反应,产物经乙酸乙酯萃取,HPLC-LSC分离测定,最后用HPLC-MS鉴定其分子结构,发现了3种与Smiles重排相关的14C标记丙酯草醚代谢物(Y1,Y2和Y3),据此推测丙酯草醚在小麦微粒体(细胞色素P450酶系)的作用下进行了羟基化。研究结果还表明,微粒体反应体系可能也有助于丙酯草醚的异构化作用。Microsomes were prepared from etiolated wheat seedlings pretreated with 5% （v/v） ethanol and 10mmol/L Pyribambenz-pmpyl for 24h at 25℃ in a dark and reacted with [C ring-U-^14C] pyribambenz-pmpyl）. Radioactive labeled compounds were extracted from the reaction solution with ethyl acetate, separated and determined by HPLC-LSC, and then identified by HPLC-MS（ESI）. Three major metabolites （Y1, Y2 and Y3 ）, presumably related to Smiles rearrangement, indicated that Pyribambenz-propyl underwent hydroxylation in wheat mierosomes and the in vitro system might also facilitate isomerization of Pyribambenz-pmpyl. Further studies are required to clarify whether rearrangement reactions of Pyribambenzpropyl is ready to occur within plant cells and whether these Smiles rearrangement-related metabolites are involved in herbicidal activities and/or detoxifieation of the （pro-） herbicide.

关 键 词：除草剂 丙酯草醚 小麦微粒体 P450s 

分 类 号：S482.4[农业科学—农药学]