自猝灭荧光探针法测定DNA  

Determination of DNA by a Self-quenching Fluorescence Probe

作　　者：赵一兵[1] 王冬媛[1] 许可慰 郭祥群[1] 许金钩[1] 

机构地区：[1]厦门大学化学系

出　　处：《高等学校化学学报》1997年第9期1456-1458,共3页Chemical Journal of Chinese Universities

基　　金：国家教育委员会博士点基金

摘　　要：This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.

关 键 词：自猝灭 荧光探针 DNA DNA探针 

分 类 号：Q523.03[生物学—生物化学]