藻胆体核心复合物结构与功能的研究(Ⅱ)──4种变藻蓝蛋白复合物发色团相互作用机制  

The Structure-Function Relationship of Core Complexes in Phycobilisome(Ⅱ) ──Studies on Chromophore-Chromophore Interactions in Four Allophycocyanin Complexes

作　　者：王慧[1] 赵井泉[1] 杨紫萱[1] 张建平[1] 蒋丽金[1] 

机构地区：[1]中国科学院感光化学研究所,北京100101

出　　处：《高等学校化学学报》1997年第9期1517-1522,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金!3970065

摘　　要：从螺旋藻藻胆体中分离出4种不同结构和光谱形式的变藻蓝蛋白复合物APⅠ、APⅡ、APⅢ和APB，利用吸收光谱、荧光光谱比较了三聚体和单体的光谱特性，通过对吸收光谱的光谱解曾以及各组分的归属，研究了变藻蓝蛋白复合物内各色团间相互作用的性质和在能量传递中的功能．结果表明，复合物内色团间的作用关系可以用Forster偶极-隅极作用机制来解释，由于连接蛋白和同源亚基的存在影响其结构的对称性，进而影响各色团间相互作用的形式和性质．Four forms of allophycocyanin (APC ) complexes with different structures andspectral properties in trinleric as well as monomeric aggregates were isolated from phycobili-some and characterized by both absorption and fluorescence spectra. The interactions be-tween chromophores of these APC complexes and their functlons in energy transfer processwere studied by deconvolutlon of absorption spectra. The results indicate that the interactionbetween chromophores of allophycocyanin complexes could be explained by Forster dipoledipole resonance Tnechanism, and it was linker polypeptides and homologous chromophoresthat influence the structural symmetry which flnally affects the interaction mechanism ofchromop hores.

关 键 词：变藻蓝蛋白 螺旋藻 藻胆体 APC PCB 发色团 

分 类 号：Q949.220.6[生物学—植物学] Q949.290.6