多巴胺转运蛋白PET成像在建立帕金森病大鼠模型中的作用  

Dopamine Transporter PET Imaging in the Evaluation of Parkinson's Disease Rat Model

作　　者：王瑞民[1] 王庆胜 郭喆[1] 张锦明[1] 尚爱加[3] 姚树林[1] 田嘉禾[1] 

机构地区：[1]解放军总医院核医学科,北京100853 [2]解放军305医院核医学科,北京100017 [3]解放军总医院神经外科,北京100853

出　　处：《中国医学影像学杂志》2008年第1期1-4,共4页Chinese Journal of Medical Imaging

基　　金：国家高科技发展研究计划(863计划2002AA205081);国家自然科学基金(60331010);解放军总医院苗圃基金资助项目(06MP076)

摘　　要：目的:研究多巴胺转运蛋白(11C-β-CFT)PET成像技术在判别帕金森病(PD)大鼠模型中的作用。材料和方法:22只成功的PD大鼠模型分别进行11C-β-CFTPET显像,ROI方法测量模型大鼠两侧纹状体/小脑比值并进行统计分析,比较两侧有无显著性差异。正常与模型大鼠分别进行TH免疫组化染色。结果:模型大鼠毁损侧纹状体放射性明显下降(P=0.000)。同时模型大鼠TH染色黑质处阳性神经元数量减少。结论:PET多巴胺转运蛋白成像可结合行为学观察作为证实模型成功的检验方法之一,为基础研究及临床诊断PD提供了一种分子成像工具。Purpose：Parkinson＇s disease（PD）rat model plays important role in pre-clinical study of PD.The purpose of this study was to verify PD model through dopamine transporter（DAT）PET imaging.Materials and Methods：Twenty-two PD rat models underwent ^11C-β-CFT PET imaging.Analysis of the striatum/cerebellum uptake ratio was performed on both side of the animals＇ brain.The imaging findings were compared with TH immunohischemical staining of midbrain tissue sections of normal rat and PD model for positive TH neurons.Results：PET imaging showed decreased ^11C-β-CFT accumulation in the lesion side of striatum.A significant difference on striatum/cerebellum ratio existed between normal and lesion sides（P = 0.000）.TH positive cells decreased in the lesion side of substantia nigra in PD model.Conclusion：DAT PET imaging,combining with behavioral observation,can be one of standards for PD model verification and a useful tool for the basic research and clinical diagnosis of PD.

关 键 词：帕金森病 帕金森病大鼠模型 多巴胺转运蛋白 PET 

分 类 号：R817[医药卫生—影像医学与核医学]