孕妇阴道乳酸杆菌种属鉴别及过氧化氢产量与妊娠结局的关系  

Identification and H_2O_2 production of vaginal lactobacilli from pregnant women and relation with outcome

作　　者：周晓梅[1] 胡晓蓉[1] 伍丽群[1] 韦瑞红[1] 刘萍 吴有利[3] 陆学东[3] 

机构地区：[1]广东医学院附属深圳福田人民医院妇产科,518033 [2]深圳市福田妇幼保健院妇产科 [3]广东医学院附属深圳福田人民医院检验科

出　　处：《中国实用医药》2008年第1期14-16,共3页China Practical Medicine

摘　　要：目的探讨孕妇阴道乳酸杆菌种属鉴别及过氧化氢产量与羊膜炎及早产的相关性。方法用16S rRNA基因序列法鉴别分离自100例20周孕龄妇女阴道拭子的乳酸杆菌种属。通过Merckoquant过氧化物试验半定量法测定各分离群的过氧化氢产量。结果77%L.jensenii L.crispatus(31%)及L.gasseri(29%)60%L.vaginalis分离群同样可产生高水平过氧化氢。乳酸杆菌分离群的过氧化氢产量越高,取样当时患者细菌性阴道病(BV)的可能性越小,分娩时羊膜炎及早产的可能性越小。结论阴道中产高水平过氧化氢乳酸杆菌的存在与孕妇BV及后来的羊膜炎及早产的风险有负相关性。可能产过氧化氢的乳酸杆菌能减少子宫上行性感染的发生率,在羊膜炎及早产发病机制中是重要的促炎症分子。Objective To explore the correlation between identification and H2O2 production of lactobacilli in vaginal from a population of pregnant women with outcomes of chorioamnionitis and preterm birth, Methods Lactobacilli were identified by partial 16S rRNA gene sequencing. H2O2 production by isolates was determined by Merckoquant Peroxide Test semiquantitative method. The antibiotic susceptibility of a representative group of strains was determined using the Antimicrobial Chemotherapy disk diffusion method guidelines. Resuits The most commonly isolated species in our study were L, crispatus, L. gasseri, L. vaginalis and L. jensenii. Amounts of H2O2 produced by lactobacilli varied widely. The presence of lactobacilli producing high levels of H2O2 was associated with a reduced risk of bacterial vaginosis and subsequent chorioamnionitis. L. jensenii and L. vaginalis produced the highest levels of H2O2. Conclusion We H2O2-producing lactobacilli are able to reduce the incidence of ascending infections of the uterus and the subsequent production of proinflammatory molecules that are important in the pathogenesis of chorioamnionitis and preterm birth.

关 键 词：乳酸杆菌 过氧化氢 妊娠结局 

分 类 号：R714.2[医药卫生—妇产科学]