补肾健脾胶囊延缓老年性耳蜗毛细胞凋亡的实验研究  

Experimental study on the preventive effect of Bu Shen Jian Pi Capsule on age-related cochlear hair cells apoptosis

作　　者：宣伟军[1] 周仲昭[2] 

机构地区：[1]华中科技大学同济医学院 [2]广西中医学院第二临床医学院,南宁530011

出　　处：《中华中医药杂志》2008年第2期114-117,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西自然科学基金资助项目(No.桂科自0447082)

摘　　要：目的:观察补肾健脾胶囊对C57小鼠老年性耳蜗毛细胞损害的保护作用并探讨其作用机制。方法:选择刚出生的C57 BL/6J小鼠30只,随机分为对照组10只,补肾健脾胶囊组20只,其中对照组小鼠为常规饲料喂养;中药组小鼠常规饲料至出生后1个月时开始饮用补肾健脾胶囊溶液以代替日常饮水。两组至7月龄时终止实验。取出小鼠耳蜗,制作成耳蜗铺片,在光学显微镜下观察并比较两组小鼠耳蜗毛细胞退行性改变状况。结果:对照组C57 BL/6J小鼠出现耳蜗毛细胞损伤加重的趋势,且这种随着年龄增长而发生的耳蜗损害遵循着从底回逐渐向顶回发展的规律,同时外毛细胞的损害比同区域的内毛细胞严重。服用复方补肾健脾胶囊的小鼠与同龄对照组小鼠相比,耳蜗毛细胞损害则相对较轻。结论:补肾健脾胶囊可以延缓C57 BL/6J小鼠随着年龄增长而发生的耳蜗毛细胞凋亡,提示该中药胶囊可能具有对抗老年性耳聋的药理效应,其作用机制可能与提高细胞环核苷酸含量,调节核酸代谢,保护和稳定DNA结构等有关。Objective： To observe the preventive effect of Bu Shen Jian Pi Capsule on age-related cochlear hair cells apoptosis in C57 BL/6J mice, and research its mechanism of action. Methods： 30 new born C57 BL/6J mice were divided into 2 groups, with 10 animals in control group （CG） and 20 animals in Bu Shen Jian Pi Capsule （experimental group, EG）. The animals in CG were fed regularly, while those in EG were fed regularly at the first one months after the birth and then, commonly fed water was substituted by the medical solution of Bu Shen Jian Pi Capsule for these animals in EG. The experimental course lasted for seven months. The cochleas of the two groups of mice were taken out for cochlear surface preparation, then being observed under optical microscope with a comparative analysis on the status of degenerative changes in the hair cells of cochlear between the tow groups. Results： Cochlear hair cells damage of CG animals showed aggravating tendency with the increase of the age, and followed the developmental rules of lesion progression in cochlear hair cells from the basal turn to the apical turn and with the number of outer hair cells loss greater than that of inner hair cells. In contrast, the cochlear damage of animals of EG was relatively milder than that in animals of CG, compared among the animals at the same age between them. Con- clusion： This study showed that Bu Shen Jian Pi Capsule could postpone age-related cochlear hair cells apoptosis in C57 BL/6J mice, therefore, it suggested that Bu Shen Jian Pi Capsule might possess the pharmacological action of preventing presbyacusis, We assume the possible protective mechanism of Bu Shen Jian Pi Capsule might be related to increase of cells cAMP, regulation of metabolism of nucleic acid, and protectoin or stabilization of DNA structure.

关 键 词：老年性耳聋 中医药疗法 补肾健脾胶囊 中药疗效 耳蜗毛细胞 

分 类 号：R285.5[医药卫生—中药学]