醋酸甲羟孕酮人工抗原的合成与鉴定  

Synthesis and identification of artificial antigen for Medroxyprogesterone Acetate

作　　者：杨泽晓[1] 张彦明[1] 韩雪清[2] 林祥梅[2] 侯义宏[1] 黄新[3] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]中国检验检疫科学研究院,北京100029 [3]清华大学化学工程系,北京100084

出　　处：《西北农林科技大学学报（自然科学版）》2008年第2期39-44,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家质检总局科研项目(2006IK017)

摘　　要：【目的】为建立醋酸甲羟孕酮(MPA)快速准确的免疫学分析技术,对MPA人工抗原的合成鉴定进行研究。【方法】将MPA肟化后与琥珀酸酐反应制得MPA半琥珀酸(MPA-HS),然后通过碳二亚胺法将MPA-HS与牛血清白蛋白(BSA)偶联合成人工抗原MPA-BSA,并采用紫外全波长扫描法、SDS-PAGE法、ELISA检测法和动物免疫试验对其进行鉴定。【结果】MPA与BSA的偶联比为8∶1时,合成的人工抗原MPA-BSA具有良好免疫原性与反应原性。【结论】成功合成了人工抗原MPA-BSA,为进一步的MPA免疫学分析技术研究奠定了基础。[Objective] This study was to develop a rapid and sensitive technology for the detection of Medroxyprogesterone Acetate（MPA）. The atrificial antigen of MPA was synthesized and identified in the study. [Method] MPA hemi hemi-succinate （MPA-HS） was produced by the oximation reaction of MPA with the reaction between oximinooximated-MPA and with succinic anhydride, then the hapten MPA-HS was conjugated to protein carrier BSA by using the coupling agent diisopropylcarbodiimide,and the synthesis synthesized antigen MPA-BSA was identified by full-wavelength the ultraviolet full wavelength scanner,SDS-PAGE,ELISA test and animal-inoculation immunization test. [Result] The results showed that the synthesis synthesized antigen MPA-BSA with good immunogenicity and reactogenicityantigenicity,and the optimized conjugation molecular ratio of coupling reaction was determined to be 8 ： 1. [Conclusion]The synthesis of artificial antigen MPA-BSA set basis for the study of the immunology detection technology of MPA.

关 键 词：醋酸甲羟孕酮 半抗原 人工抗原 合成与鉴定 

分 类 号：Q939.91[生物学—微生物学] S851[农业科学—预防兽医学]