雷公藤多甙纳米乳的抗炎及免疫抑制试验  

Study on anti-inflammatory and immunoinhibition test of Tripterygium polyglycosides nanoemulsion

作　　者：何欣[1] 欧阳五庆[1] 许利耕[1] 阳卫超[1] 吴旭锦[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第2期67-71,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家科技支撑计划项目(2006BAD04A11);西北农林科技大学校长基金项目(130709)

摘　　要：【目的】制备雷公藤多甙纳米乳(TP-NE),并考察其抗炎及免疫抑制效果。【方法】滴定法制备雷公藤多甙纳米乳,建立SD大鼠蛋清性关节炎和佐剂性关节炎模型,用雷公藤多甙片(TP)和TP-NE进行治疗,治疗过程中测量足部体积,观察其抗炎效果,治疗24 d后用噻唑兰(MTT)法研究TP-NE对脾淋巴细胞增殖活性的影响,并检测血清中超氧化物歧化酶(SOD)的活性和氧化氮(NO)的含量。【结果】TP-NE棕黄色、澄清、透明、平均粒径为23.6 nm,其对大鼠蛋清性关节炎有保护作用,对大鼠佐剂性关节炎有治疗作用,且给药时间越长治疗效果越显著;TP-NE对脾淋巴细胞增殖有显著的抑制作用,可以显著增强血清中SOD活性,极显著降低血清中NO含量。【结论】TP-NE具有较好的抗炎和免疫抑制效果,且具有一定的缓释效应。[Objective] Tripterygium polyglycoside nanoemulsion（TP-NE） was prepared to asses its anti-inflammatory and immunoinhibition effects. [Method] TP-NE was prepared by titrimetric method,albumen arthritis and adjuvant-induced arthritis were cured by TP and TP-NE,the anti-inflammatory effects by measuring the volume of foot each 4 d during the curing period was researched. After 24 d,the multiplication activity of splenic lymphocyte through MTT method, was observeal and the content of saperoxide dismutase （SOD） and nitrogen manoxidum （NO） in serum was detected. [Result] The results showed that the TP-NE was clear, the mean diameter was 23.6 nm. The albumen arthritis could be prevented and the inflammatory reaction of adjuvant-induced arthritis could be relieved lympholeukocyte proliferation could be inhibited remark- ably. The activity of SOD was reinforced and the content of NO was lowered in serum. [Conclusion] TP-NE has preferably anti-inflammatory and immunoinhibition effect and has slow-release potency.

关 键 词：纳米乳 雷公藤多甙 抗炎 免疫抑制 

分 类 号：S853.76[农业科学—临床兽医学]