Baculovirus per os Infectivity Factors Are Involved in HearNPV ODVs Infection of HzAM1 Cells in vitro  

作　　者：Ting JIANG Xiang LI Jian-hua SONG Chang-yong LIANG Xin-wen CHEN 

机构地区：[1]State Key Laboratory of Virology, Wuhan Institute of Virology, the Chinese Academy of Sciences, Wuhan 430071, China [2]Shanghai Public Health Center Affiliated to Fudan University, 56 Shui Dian Road, Shanghai, China.

出　　处：《Virologica Sinica》2008年第1期25-30,共6页中国病毒学（英文版）

基　　金：National Nature Science Foundations ofChina (30325002, 30470075);National Basic ResearchPriorities Program of China (2003CB1140).

摘　　要：Baculoviruses produce two viral phenotypes, the budded virus （BV） and the occlusion-derived virus （ODV）. ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. Here we present data demonstrating that Helicoverpa armigera nucleopolyhedrovirus （HearNPV） ODV infected cultured Hz-AM1 cells in a pH dependent manner. The optimal pH for ODV infection was 8.5, which is same to that in the microvilli of midgut epithelial cells, the ODV native infection sites. Antibodies neutralization analysis indicated that four HearNPV oral infection essential genes p74, pif-l, pif-2 and pif-3 are also essential for HearNPV ODV infection in vitro. Thus, HearNPV-HzAM1 system can be used to analyze the mechanism of ODV entry.Baculoviruses produce two viral phenotypes, the budded virus (BV) and the occlusion-derived virus (ODV). ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. Here we present data demonstrating that Helicoverpa armigera nucleopolyhedrovirus (HearNPV) ODV infected cultured Hz-AM1 cells in a pH dependent manner. The optimal pH for ODV infection was 8.5, which is same to that in the microvilli of midgut epithelial cells, the ODV native infection sites. Antibodies neutralization analysis indicated that four HearNPV oral infection essential genes p74, pif-1, pif-2 and pif-3 are also essential for HearNPV ODV infection in vitro. Thus, HearNPV-HzAM1 system can be used to analyze the mechanism of ODV entry.

关 键 词：Helicoverpa armigera nucleopolyhedrovirus （HearNPV） Occlusion derived virus （ODV） per osinfectivity factor（pif） p74： Baculovirus 

分 类 号：Q939.4[生物学—微生物学]