血管内皮生长因子和血小板反应素在卵巢上皮癌中的表达及其意义  

Expression of vascular endothelial growth factor and thrombospodinl in epithelial ovarian cancer and their correlations with tumor angiogenesis and prognosis

作　　者：谭先杰[1] 郎景和[1] 楼伟珍[1] 沈铿[1] 许秀英[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,100730

出　　处：《中华医学杂志》2008年第5期293-298,共6页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30100199）;北京科技新星计划基金资助项目（2003A25）

摘　　要：目的探讨测血管内皮生长因子（VEGF）和血小板反应素1（TSP1）蛋白质在卵巢上皮癌中的表达与肿瘤微血管密度（MVD）的关系及其临床意义。方法用免疫组织化学法检测57例原发性卵巢上皮癌组织和22例正常卵巢组织中VEGF和TSP1蛋白质的表达，并用CD34抗体免疫染色后进行MVD计数。结合随诊资料，对上述指标与卵巢上皮癌临床病理学特征及3年存活率之间的关系进行回顾分析。结果卵巢上皮癌组织中VEGF蛋白质的阳性表达率高于正常卵巢组织（分别为70．2％和45．5％，P〈0．05），且多为强阳性表达。卵巢上皮癌中组织中TSP1多为弱阳性表达，总阳性表达率低于正常卵巢组织（分别为47．4％和72．7％，P〈0．05）。卵巢上皮癌组织的MVD显著高于正常卵巢组织（分别为30士9和20士8，P〈0．05）。VEGF阳性和TSP1阴性与卵巢上皮癌的手术病理学分期晚、大网膜转移和癌性腹水显著相关。VEGF的表达与MVD呈正相关（P〈0．01），TSP1的表达与MVD呈负相关（P〈0．01）。VEGF阳性和TSP1阴性均与患者3年存活率呈负相关。VEGF阳性而TSP1阴性组的3年存活率明显低于VEGF阴性而TSP1阳性组（分别为24．0％和83．3％，P〈0．01）。结论卵巢上皮癌组织VEGF和TSP1蛋白质的表达与肿瘤的血管形成有关，对上述分子的检测可帮助判断卵巢上皮癌的生物学行为，从而提供预后信息。Objective To evaluate the expression of vascular endothelial growth factor （VEGF） and thrombospodinl （TSP1） in epithelial ovarian cancer and their correlations with tumor angiogenesis and prognosis. Methods Fifty-seven samples of primary epithelial ovarian cancer and 22 samples of normal ovary from patients with hysteromyoma obtained during operation underwent immunohistochemistry with anti- VEGF, anti-TSP1, and anti-CD34 antibodies. Microvessel density （MVD） in the tumor tissue was evaluated on the base of CD-34 immunostaining. The correlations of VEGF and TSP1 expression with clinico-pathologic characteristics and 3-years survival rates were analyzed retrospectively. Results The positive rate of VEGF protein of the epithelial ovarian cancer was 70.2%, significantly higher than that of the normal ovarian tissue （45.5%, P 〈 0.05 ）. The positive rate of TSP1 protein of the epithelial ovarian cancer was 47.4%, significantly lower than that of the normal ovarian tissue （ 72.7%, P 〈 0.05 ）. The MVD of the epithelial ovarian cancer was （30 ± 9）, significantly higher than that of the normal ovarian tissue （ 20 ± 8, P 〈 0. 05 ）. The VEGF positive expression and TSP1 negative expression were positively correlated with the FIGO stage, omentum metastases, and malignant ascites （ P 〈 0. 01, 〈 0. 01, and 〈 0. 05 respectively）. VEGF expression was positively correlated with MVD （ P 〈 0.01 ）, and TSP1 expression was negatively correlated with MVD （ P 〈 0.01 ）. Increased expression of VEGF and decreased expression of TSP1 with were reversely correlated with the patients＇ survival. The 3-year survival rate of the patients positive in VEGF and negative in TSP1 was 24.0%, significantly lower than that of the patients negative in VEGF and positive in TSP1 （83.3%, P 〈 0.01 ）. Conclusion Increased expression of VEGF and decreased expression of TSP1 in epithelial ovarian cancer are closely related to its angiogenesis. Detection of these two proteins contributes to ident

关 键 词：卵巢肿瘤 生长物质 反应素类 血管 

分 类 号：R686[医药卫生—骨科学]