姜黄素对离体培养大鼠海马神经元突触传递的影响  

Effects of curcumin on the synaptic transmission of cultured hippocmnpal neurons of rats

作　　者：杜鹏[1] 郭曦[1] 彭伟锋[1] 马昱[1] 范薇[1] 汪昕[1] 

机构地区：[1]复旦大学附属中山医院神经内科,上海200032

出　　处：《中华医学杂志》2008年第5期333-336,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30470593）;复旦大学青年科学基金资助项目（2005年）

摘　　要：目的探讨姜黄素对离体培养的大鼠海马神经元突触传递的影响。方法离体培养胚胎18dSD大鼠海马神经元，9d后加入姜黄素（终浓度10μmol／L），继续培养24h，采用全细胞膜片钳技术，自由记录模式，观察姜黄素对海马神经元自发兴奋性突触后电流（sEPSC）和微小兴奋性突触后电流（mEPSC）频率和幅度的影响。结果（1）姜黄素干预的海马神经元sEPSC频率（1．4Hz±0．5Hz）明显高于对照组（0．8Hz±0．4Hz，P〈0．01）；但姜黄素组海马神经元sEPSC幅度（1834pA±244pA）和对照组海马神经元sEPSC幅度（1721pA±391pA）比较差异没有统计学意义（P=0．115）。（2）姜黄素干预的海马神经元mEPSC频率（6．4Hz±0．8Hz）明显高于对照组（5.1Hz±0．9Hz，P〈0．01）；但姜黄素组神经元mEPSC幅度（34pA±10pA）和对照组海马神经元mEPSC幅度（30pA±9pA）比较差异没有统计学意义（P=0．057）。结论姜黄素可以增强海马神经元突触传递，这可以为最近研究发现姜黄素具有缓解脑缺血、改善AD大鼠认知功能提供神经电生理学依据。【Objective To explore the effects of curcumin on the synaptic transmission of the cultured hippocampal neurons of rats. Methods Hippocampal neurons were obtained from 18-day-old fetal SD rats, cultured for 9 days, and then divided into 2 groups ： curcumin group, with curcumin of the final concentration of 10 μmol/L added into the culture fluid for 24 h, and control group with DMSO added into the cultured fluid. Simultaneous excitatory postsynaptic current （sEPSC） and miniature excitatory postsynaptic current （mEPSC） of the rat hippocampal neurons in these two groups were observed using the method of patch clamp, whole cell recording, and gap free mode. Results （ 1 ） The frequency of sEPSC in the neurons treated with curcumin was 1.4 Hz ± 0.5 Hz, significantly higher than that of the control group （0.8 Hz ±0.4 Hz, P 〈 0.01 ）. The amplitude of sEPSC of the neurons treated with curcumin was 1834 pA ±244 pA, not significantly different from that of the control group （1721 pA ±391 pA, P = 0. 115）. （2） The frequency of mEPSC in the neurons treated with curcumin was 6.4 Hz ± 0.8 Hz, significantly higher than that of the control group （5.1 Hz±0.9 Hz, P 〈 0. 001 ） . The amplitude of mEPSC in the neurons treated with curcumin was 34 pA ± 10 pA. Not significantly different from that in the neurons treated with DMSO （ 30 pA ± 9 pA, P = 0. 057 ）. Conclusion Curcumin modulates or enhances the synaptic transmission, which possibly contributes to the cognition enhancing effect of curcumin in rats suffering from cerebral ischemia.

关 键 词：姜黄素 海马神经元 突触传递 膜片钳 

分 类 号：R686[医药卫生—骨科学]