射频电磁场对人树突状细胞的表型和功能影响的体外研究  

Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields

作　　者：周智东[1] 曾群力[2] 郑云[1] 张建宾[1] 陈海祥[1] 鲁德强[2] 邵传森[1] 夏大静[1] 

机构地区：[1]浙江大学医学院免疫学研究所 [2]浙江大学医学院浙江省生物电磁学重点研究实验室,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2008年第1期29-33,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目(30471588)

摘　　要：目的:研究手机射频电磁辐照对人树突状细胞(DC)的表型和功能的影响,为评价手机射频电磁场对免疫系统功能的影响提供实验依据。方法:用比吸收率(SAR)为4 W/kg的1800 MHzGSM射频电磁场间断(5 min开10 min停模式)辐照DC细胞0 h、1 h、12 h或24 h,以流式细胞仪检测各处理组DC表面HLA-DR和协同刺激分子(CD80、CD86、CD40及CD11c)的变化;以CCK-8试剂检测各组DC的同种混合淋巴细胞反应(MLR)能力的差异;以ELISA双抗体夹心法检测各组DC分泌细胞因子IL-12p70、TNF-α功能的差异。结果:与假辐照组相比,各辐照处理组DC表型分子HLA-DR、CD80、CD86、CD40的阳性细胞百分率均明显下降(P<0.05),但CD11c无明显变化;各辐照处理组DC的同种MLR能力明显降低(P<0.05),尤其以24 h处理组为甚。各辐照处理组DC的IL-12p70、TNF-α的分泌无显著差异。结论:在本实验条件下,射频电磁场辐照对DC表面分子的表达及对同种T细胞的刺激能力具有一定的抑制作用。Objective： To investigate the effects of mobile phone 1800 MHz electromagnetic fields （EMF） on the surface markers and the functions of human dendritic cells （DC）. Methods： Human DCs were exposed to intermittent 5 rain on/10 rain off EMF with specific absorption rates （SAR） 4 W/kg for 0 h, 1 h,12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CDS0,CD86,CD40 and CDllc. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction （allo-MLR） of DC,and enzyme linked immunosorbent assay （ELISA） to identify the levels of IL-12p70 and TNF-α secretedby DC. Results： Compared with the sham radiation group,after exposure to the electromagnetic fields for 1 h, 12 h,or 24 h,HLA-DR, CDS0, CD86 and CD40 were all declined except CDllc. The ability of DC allo-MLR in each exposure group was decreased significantly （P〈0.05）,especially in the 24 h exposure group. However,the secreted levels of IL-12p70 and TNF-α of DC in each exposure group remained no changed. Conclusion： The study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.

关 键 词：树突细胞/免疫学 电磁场/副作用 淋巴细胞培养试验 混合 细胞因子类 细胞 培养的 表型 

分 类 号：R392[医药卫生—免疫学]