强直性脊柱炎患者血清可溶性肿瘤坏死因子相关凋亡诱导配体的表达及其临床意义  

Expression of serum soluble TNF-related apoptosis-inducing ligand in patients with ankylosing spondylitis and its clinical significance

作　　者：杨再兴[1] 梁艳[1] 朱烨[1] 李畅[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科,上海200003

出　　处：《第二军医大学学报》2008年第1期68-71,共4页Academic Journal of Second Military Medical University

基　　金：国家高技术研究发展计划("863"计划)课题(2006AA02Z496);国家自然科学基金(30471616)~~

摘　　要：目的:探讨血清可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)在强直性脊柱炎(AS)患者、类风湿性关节炎(RA)患者及健康对照间的表达差异及其临床意义。方法:60例AS患者按HLA-B27抗原测定结果分为阳性38例,阴性22例;按Bath活动指标分为活动组41例,非活动组19例;另设20例RA患者和30例健康体检者作为对照。应用ELISA法检测上述研究对象血清sTRAIL的浓度,应用ESR自动化分析仪和特定蛋白分析仪测定红细胞沉降率(ESR)和血清C反应蛋白(CRP)含量。结果:HLA-B27阳性和阴性AS患者血清sTRAIL无统计学差异,均明显高于RA组和健康对照组(P<0.01);活动组AS患者血清sTRAIL水平明显高于非活动组(P<0.01);HLA-B27阳性AS患者血清sTRAIL水平与CRP呈明显相关(r=0.609,P=0.000),而阴性患者无显著相关;HLA-B27阳性和阴性AS患者血清sTRAIL与ESR均无明显相关。结论:血清sTRAIL水平在AS中明显上调,且与HLA-B27状态无关,但AS患者血清sTRAIL与CRP的相关性受HLA-B27状态的影响。Objective： To investigate the differential expression of serum soluble TNF-related apoptosis-inducing ligand （sTRAIL） between ankylosing spondylitis （AS） and rheumatoid arthritis patients （RA） and to discuss its clinical significance. Methods： Sixty AS patients, including 38 HLA-B27-positive ones and 22 HLA-B27-negative ones, 20 rheumatoid arthritis （RA） patients and 30 healthy individuals were included in the present study, The AS patients were divided into active group and inactive group based on bath ankylosing spondylitis disease activity index （BASDAI）. The concentrations of serum sTRAIL were measured by ELISA in all groups, Erythrocyte sedimentation rate （ESR） and C-reactive protein （CRP） were detected automatically by ESR automatic analyzer and specific protein analyzer, Results： The serum sTRAIL concentration was significantly higher in AS patients （both H LA-B27-positive and -negative AS patients） than in RA patients and healthy controls （P〈0, 01） ; no significant difference was found between HLA-B27-positive and -negative AS patients, Serum sTRAIL concentration was significantly higher in active AS group than in inactive AS group（P〈0, 01）. Serum sTRAIL and CRP concentrations were correlated with each other in HLA-B27-positive AS patients（r= 0, 609, P= 0. 000）, but not in HLA-B27-negative ones. Serum sTRAIL concentration was not correlated with ESR in AS patients, Conclusion,. Serum sTRAIL is obviously up-regulated in AS patients, which is not associated with the status of HLA-B27. However, the association between sTRAIL and CRP is influ- enced by the status of HLA-B27.

关 键 词：强直性脊柱炎 类风湿性关节炎 可溶性肿瘤坏死因子相关凋亡诱导配体 HLA—B27抗原 

分 类 号：R593.23[医药卫生—内科学]