2个山羊品种多胎性状的微卫星标记研究  

Studies on Microsatellite Markers of Fecundity Trait in Two Goat Breeds

作　　者：宋宇轩[1] 朱广琴[1] 王益兵[1] 王建刚[1] 曹斌云[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100

出　　处：《畜牧兽医学报》2008年第1期16-23,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术研究发展计划(863计划)项目(2007AA10Z167);西北农林科技大学校青年科研专项(04ZM004);国家"十一五"奶业重大科技支撑项目(2006BAD04A11)

摘　　要：根据比较基因组学原理,选择与绵羊高繁殖力紧密关联的4个微卫星标记OarAE101、OarHH55、BM1329、BMS2508,分析其在波尔山羊和西农莎能奶山羊中的多态性分布情况。结果表明,4个微卫星标记在波尔山羊中的多态信息含量(PIC)分别为0.834 0、0.722 2、0.802 1、0.685 5,有效等位基因数(Ne)分别为6.719、4.098、5.610、3.542;在莎能奶山羊中的PIC分别为0.765 5、0.738 7、0.670 1、0.682 0,Ne分别为4.849、4.360、3.520、3.609。4个微卫星基因座对波尔山羊和莎能奶山羊的产羔数效应分析表明,在波尔山羊群体中,OarAE101基因座126 bp等位基因对产羔数具有正效应,而134 bp则呈负效应;OarHH55基因座120和124 bp等位基因对产羔数具有正效应,而130 bp/140 bp基因型则呈负效应;BM1329基因座185 bp等位基因对产羔数具有正效应,而176 bp等位基因和215 bp/240 bp基因型均呈负效应;BMS2508基因座118 bp等位基因对产羔数具有正效应,而122 bp/141 bp基因型和148 bp等位基因均呈负效应。在西农莎能奶山羊群体中,OarAE101基因座108 bp等位基因和114 bp/126 bp基因型对产羔数均呈正效应,而124 bp等位基因则呈负效应;OarHH55基因座120 bp等位基因对产羔数呈正效应,而150 bp/165 bp基因型呈负效应;BM1329基因座185 bp等位基因对产羔数呈正效应,而176 bp等位基因和215 bp/240 bp基因型则均呈负效应;BMS2508基因座118 bp等位基因对产羔数呈正效应,而141 bp等位基因和153 bp/160 bp基因型均呈负效应。According to comparative genomics,four microsatellite loci including OarAE101,OarHH55,BM1329 and BMS2508 that linked to the fecundity gene FecB in the sheep chromosome 6 were analyzed for polymorphisms in Boer goat and Xinong Shaanen goat.The results indicate that there are genetic polymorphisms at these 4 microsatellite loci.The polymorphism information content（PIC） and number of effective alleles（Ne） are 0.834 0,0.722 2,0.820 1,0.685 5 and 6.719,4.098,5.610,3.542 respectively in Boer goats;the PIC and Ne are 0.765 5,0.738 7,0.670 1,0.682 0 and 4.849,4.360,3.520,3.609,respectively in Xinong Shaanen goats.The result of the effect of the 4 microsatellite loci on the litter size of 2 goat breeds showed that the allele 126 bp at OarAE101 has positive effects on the litter size in Boer goat but 134 bp has negative effects;the allele 120 bp and 124 bp at OarHH55 have positive effects on the litter size in Boer goat,but 130 bp/140 bp has negative effects;the allele 185 bp at BM1329 has positive effects on the litter size in Boer goat but 176 bp,215 bp/240 bp have negative effects;the allele 118 bp at BMS2508 has positive effects on the litter size in Boer goat and 122 bp/141 bp,148 bp has negative effects;the allele 108 bp,114 bp/126 bp at OarAE101 have positive effects on the litter size in Xinong Shaanen goat and 124 bp has negative effects;the allele 120 bp at OarHH55 has positive effects on the litter size in Xinong Shaanen goat and 150 bp/165 bp has negative effects;the allele 185 bp at BM1329 has positive effects on the litter size in Xinong Shaanen goat and 176 bp,215 bp/140 bp have negative effects;the allele 118 bp at BMS2508 has positive effects on the litter size in Xinong Shaanen goat and 141 bp,153 bp/160 bp have negative effects.

关 键 词：微卫星标记 山羊 遗传多样性 产羔数 

分 类 号：S827.2[农业科学—畜牧学]