鸡肌内脂肪双向选择对脂肪性状及相关基因mRNA表达的影响  

Studies on the Differences of Phenotypic Values and mRNA Expression of Genes Associated with Fat Deposition in the Divergent-selected Chicken Lines

作　　者：李冬立[1] 文杰[1] 赵桂苹[1] 郑麦青[1] 陈继兰[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《畜牧兽医学报》2008年第1期24-28,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30771546);国家科技支撑计划(2006BAD01A00);"863"计划(2006AA10Z1D8)

摘　　要：本试验旨在研究鸡肌内脂肪(IMF)双向选择对脂肪性状及相关基因mRNA表达的影响。试验采用北京油鸡肌内脂肪双向选育系和对照系雏鸡,饲养至56和120 d时,每个系分别屠宰30只母鸡,测定胸肌和腿肌IMF含量、腹脂重、腹脂率及LPL、H-FABP和A-FABP基因mRNA表达量,比较双选系各性状差异,分析IMF遗传选择效果。结果表明,上选系肌肉IMF含量和肉色亮度值(L*)显著大于下选系(P<0.05),胸肉终pH(pHu)显著低于下选系(P<0.05)。LPL基因在120日龄上选系腹脂中的mRNA表达量显著高于下选系(P<0.05),下选系胸肌中H-FABP基因mRNA的表达显著高于上选系(P<0.05)。结果表明IMF的双向选择是有效的,该双选系可以作为未来基因定位和基因互作效应研究的宝贵资源群体。Three groups of chickens were fed to study the differences of phenotypic values and mRNA expression of genes associated with fat deposition in the divergent-selected chicken lines.The IMF divergent-selected lines and unselected Beijing You chicken were fed and slaughtered at day 56 and 120.The phenotypic values of IMF content,abdominal fat weight,abdominal fat proportion and mRNA expression of LPL,H-FABP and A-FABP genes were measured and compared among groups.The results showed that IMF content and L^＊ value of chicken muscle in up-selected line（UL） was higher than the down-selected line（DL） at both day of 56 and 120,but the ultimate pH of breast muscle was lower.The differences were significant（P〈0.05）.At day of 120,the LPL in abdominal fat of UL expressed more than DL significantly（P〈0.05）,but the H-FABP in breast muscle expressed less significantly（P〈0.05）.

关 键 词：鸡 IMF双向选育系 IMF MRNA表达 

分 类 号：S831.2[农业科学—畜牧学]