肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化  

Regional and Ontogenetic Expression of NHE2 mRNA in Intestine of Broiler

作　　者：王修启[1] 邹仕庚[1] 左建军[1] 苏海林[1] 黄志毅[1] 冯定远[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《畜牧兽医学报》2008年第1期42-47,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(973)项目(2004CB117501);国家自然科学基金(30671519)

摘　　要：选用遗传背景相同的1日龄父母代雄性Arbor Acre（AA）肉雏鸡120羽,随机分为4个重复,采用相对定量RT-PCR方法,以30日龄AA肉鸡肠道RNA为模板,研究肉鸡肠道钠/氢交换载体2（Sodium hydrogen exchanger 2,NHE2）mRNA表达的组织特异性;以AA肉鸡十二指肠和空肠RNA为模板,研究肉鸡肠道NHE2mRNA表达的发育性变化。结果显示：①AA肉鸡十二指肠和空肠NHE2 mRNA的表达丰度显著高于回肠和结直肠（P〈0.05）,而十二指肠和空肠之间、回肠和结直肠之间无显著差异（P〉0.05）;②AA肉鸡NHE2 mRNA在十二指肠及空肠中的表达具有相同的发育模式,2～16日龄升高,30～44日龄下降,55日龄略微回升;在16和30日龄时的表达丰度显著高于2、44和58日龄（P〈0.05）。以上结果说明：AA肉鸡肠道近端NHE2 mRNA的表达丰度显著高于远端（P〈0.05）。AA肉鸡十二指肠及空肠NHE2 mRNA的表达具有相同的发育模式,表明NHE2mRNA表达受到发育阶段的调控,且在十二指肠和空肠间具有稳定性。120 1-day-old parental male Arbor Acre（AA） broilers were randomly divided into 4 replicates,respectively.Regional expression of sodium hydrogen exchanger 2（NHE2） mRNA in different intestinal tract segments of AA broiler at 30-day-old and ontogenetic expression of NHE2 mRNA in duodenum and jejunum of AA broilers were determined by relative quantitative RT-PCR.It was found that：（1） Expression of NHE2 mRNA in duodenum,jejunum,ileum and colorectum was declined.The abundance of NHE2 mRNA in duodenum and jejunum was higher than that in ileum and colorectum（P〈0.05）,respectively.（2）Ontogenetic patterns of NHE2 mRNA were same between duodenum and jejunum.The abundance of NHE2 mRNA was increased from 2-to 16-day-old,decreased from 30-to 44-old-day and increased again on 58-day-old.The abundance of NHE2 mRNA on 16-and 30-day-old were higher than that on 2-,44-and 58-day-old（P〈0.05）,respectively.These results indicate that regional expression of NHE2 mRNA in intestine of AA broilers was significantly declined along the downward intestine from proximal intestine to distal intestine（P〈0.05）.Ontogeny of NHE2 mRNA in AA broilers had the same pattern in duodenum and jejunum,indicating that NHE2 mRNA expression could be regulated by developmental stage.

关 键 词：肉鸡 肠道 NHE2 MRNA 组织特异性 发育性表达 

分 类 号：S831.2[农业科学—畜牧学]