复合黏膜免疫佐剂对鸡小肠细胞免疫的影响  

Effects of Compound Mucosal Immune Adjuvants on Cellular Immune Responses of Small Intestine in Chicken

作　　者：张小飞[1] 杨倩[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,南京210095

出　　处：《畜牧兽医学报》2008年第1期85-90,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30070564);江苏省科技厅农业科技攻关项目(BE2004318)

摘　　要：分别应用复合黏膜免疫佐剂Ⅰ和Ⅱ与新城疫Ⅳ系弱毒苗混合(佐剂Ⅰ组和佐剂Ⅱ组),经口免疫鸡后,研究鸡小肠黏膜局部CD3+T细胞、上皮内淋巴细胞和干扰素-γ(Interferon-γ,IFN-γ)mRNA表达水平的变化。结果表明,在整个免疫期内CD3+T细胞和上皮内淋巴细胞数量都呈上升趋势,在首免后第3和第5周时十二指肠CD3+T细胞佐剂Ⅱ组比佐剂Ⅰ组显著增加(P<0.05),空肠CD3+T细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加(P<0.01),第7周时Peyer’s斑上皮内淋巴细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加(P<0.01);整个免疫期内佐剂Ⅱ组和佐剂Ⅰ组IFN-γmRNA表达水平呈下降趋势,表达量较低。本研究表明2种复合黏膜免疫佐剂都能明显增加黏膜局部免疫细胞的数量,提高小肠局部黏膜免疫力。The chicken were immunized orally with NDV strain LaSota vaccine containing two kinds of compound mucosal immune adjuvant（Group adjuvant Ⅰ and Ⅱ） respectively.The CD3^＋T lymphocytes,intraepithelial lymphocytes（IEL） and IFN-γ mRNA expression of small intestine in chicken were investigated.The results showed that the number of CD3^＋T lymphocytes and IELs increased during the period of immunity.The number of CD3^＋T lymphocytes of Group adjuvant Ⅱ were higher than that of Group adjuvant Ⅰ（P〈0.05） in duodenum and significantly higher（P〈0.01） in jejunum at the 3rd week and 5th week after vaccination.The number of IELs of Group adjuvant Ⅱ were significantly higher than that of Group adjuvant Ⅰ（P〈0.01） in Peyer＇s Patch at the 7th week after vaccination.The IFN-γ mRNA expression of two compound adjuvant groups decreased after vaccination.The results demonstrated that compound adjuvants could induce high level cellular immune response and promote intestine mucosal immunity in chicken.

关 键 词：复合黏膜免疫佐剂 CD3^+T细胞 上皮内淋巴细胞 干扰素-Γ 

分 类 号：R392.1[医药卫生—免疫学] S852.4[医药卫生—基础医学]