遗传距离与利用微卫星进行系统树构建初探  

The Relationship between Construction of Phylogenetic Trees and Genetic Distances from Microsatellite

作　　者：毛永江[1] 常洪[1] 杨章平[1] 张柳[1] 许明[1] 常国斌[1] 孙伟[1] 宋光明[1] Henner Simianer 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]Institute of Animal Breeding and Genetics,University of Goettingen,Goettingen 37075,Germany

出　　处：《畜牧兽医学报》2008年第2期129-135,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“863”计划(2002AA24211);国家自然科学基金(30571323);国家留学基金委资助项目

摘　　要：以中国牛亚科家畜6个群体12对微卫星数据为基础,采用6种遗传距离(DA、DC、DS、Dsw,(σμ)2和DTL)和2种聚类方法(UPGMA和NJ法),探索它们对系统树构建准确性的影响。结果表明:微卫星位点平均杂合度水平显著影响系统树构建的准确性,用DA和DC遗传距离构建的系统树准确性相对较高,但很难确定NJ法和UPGMA法哪一种更适合于系统树的构建,而DTL遗传距离用于系统树构建有待进一步完善;最后,对微卫星用于家畜系统树构建的有效性和局限性进行了探讨。6 genetic distances （DA, DC, DS, DSW, （σμ）^2 and DTL ） and 2 cluster methods （UPGMA and NJ） were used to evaluate the reliabilities of construction of phylogenetic trees based on the microsatellite data from 6 cattle populations in bovine subfamily in China. The results showed that the reliabilities of construction of phylogenetic trees were affected significantly by the heterozygosity of microsatellite sites. DA and Dc genetic distances showed generally higher reliability than other distances to obtain correct phylogenetic tree, but we can＇t confirm which is better to obtain the correct phylogenetic tree for NJ and UPGMA. DTL genetic distance needed to be improved to construct the phylogenetic tree. Finally, usefulness and limitations were discussed to construct of phylogenetic trees from microsatellites.

关 键 词：系统树构建 微卫星 遗传距离 

分 类 号：S823.2[农业科学—畜牧学]