东亚和南亚固有绵羊群体X-蛋白等位基因特有频率分布的研究  

Study on the Specific Frequency Distribution of Xprotein Alleles in Indigenous Sheep Populations in East and South Asia

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作  者:孙伟[1] 常洪[1] Tsunoda Kenji 杨章平[1] 张鉴华[1] 马国龙[1] 鲁生霞[1] 杜垒[1] 

机构地区:[1]扬州大学动物科学与技术学院,扬州225009 [2]日本昭和大学医学部,东京142-8555

出  处:《畜牧兽医学报》2008年第2期143-149,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基  金:国家自然基金国际合作项目(30310103007;30410103150);江苏省自然科学基金(BK2007556);江苏省教育厅基金项目(NK051039);扬州大学“新世纪人才工程”优秀骨干教师项目基金资助

摘  要:本研究利用单向水平板淀粉凝胶电泳以我国5个固有地方绵羊品种包括湖羊(Hu)、同羊(Tong)、滩羊(Tan)、小尾寒羊(Han)、洼地羊(WD)为研究对象对X-蛋白遗传多样性进行了检测,并引用我国周边国家、地区绵羊品种为分析背景。结果表明以喜马拉雅山脉为界的亚洲东部和南部的北部群体和南部群体在编码的X(+)型的显性等位基因X频率上存在极显著差异(P〈0.01)。北部检测的群体中X等位基因频率范围为0~0.18,平均值为0.0878,包括属于藏羊和蒙古羊系统的小尾寒羊(Han)、不丹羊(Bhutan)、同羊(Tong)、湖羊(Hu)、滩羊(Tan)、洼地绵羊(WD)、Bhyanglung绵羊(Bhy)、Baruwal绵羊(Bar)、云南绵羊(Yunnan)、哈拉和林绵羊(Kh)和乌兰巴托绵羊(Ub)。南部检测的群体中X等位基因频率范围为0.2037~0.4655,平均值为0.3082,包括属于印度绵羊系统的孟加拉国绵羊(Ban)、Kagi绵羊(Kagi)、Lampuchhre绵羊(Lamp)、越南占部落绵羊(Cham)和缅甸绵羊(Mya)。本研究揭示X等位基因可作印度绵羊的标记,在绵羊群体尤其是亚洲东部和南部绵羊系统形成的研究中具有潜在的重要作用。The X-protein polymorphism of five Chinese native sheep populations including Hu sheep (Hu), Tong sheep (Tong), Tan sheep (Tan), Small-tailed Han sheep (Han) and Wadi sheep (WD) were determined using one-dimensional and horizontal starch gel electrophoresis, with other 10 Asian native sheep populations around China as the analyzed background. The result showed that an extreme significant difference in the frequency of the X allele coding dominantly for the X (+) type between the northern and southern populations of native east and south of Asia sheep divided by the boundary of the Himalaya Mountains was seen (P〈0.01). The X allele frequency ranged from 0 to 0.18 with an average of 0. 087 8 in the northern population examined, consisting of Small tailed Han sheep (Han), Bhutan sheep (Bhutan), Tong sheep (Tong), Hu sheep (Hu), Tan sheep (Tan), Wadi sheep (WD), Bhyanglung sheep (Bhy), Ba-ruwal sheep (Bar), Yunnan sheep (Yunnan), Khalkhas sheep (Kh) and Ulaanbaatar sheep (Ub) belonging to the Tibetan and Mongolian sheep groups. In contrast, the frequency of the same allele was in the range of 0. 203 7-0. 465 5 and the mean frequency was 0. 308 2 in the southern population tested, consisting of the Ban (Bangal sheep), Kagi (Kagi sheep), Lamp (Lampuchhre sheep), Cham (Vietnamese Cham sheep) and Mya (Myanmar sheep), which belong to the Indian sheep group. This finding suggests that the X allele appears to be an Indian sheep marker and is potenitially important in phylogenetic studies on native sheep populations, especially in east and south of Asia

关 键 词:X-蛋白 遗传多样性 东亚和南亚固有绵羊 系统发生基因标记 

分 类 号:S826.2[农业科学—畜牧学]

 

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