H9N2亚型禽流感病毒复制特性的基因分析  

Analysis of Genes Affecting the Replication Property of H9N2 Avian Influenza A Viruses

作　　者：石火英[1] 孙蕾[1] 陈素娟[1] 卢建红[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《畜牧兽医学报》2008年第2期189-194,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省属高校重大基础研究项目资助(05KJA23016);国家自然科学基金项目资助(30670079)

摘　　要：利用反向遗传技术,通过基因重排方法,以A/Chicken/Shanghai/F/98(H9N2)禽流感病毒(Avian influ-enza virus,AIV)的6个内部基因为骨架,与A/Chicken/Guangdong/SS/94(H9N2)AIV的HA和NA基因组合,产生3株H9N2亚型重排AIVs。动物试验发现A/Chicken/Shanghai/F/98(H9N2)和A/Chicken/Guangdong/SS/94(H9N2)AIV主要在呼吸系统复制,A/Chicken/Shanghai/F/98(H9N2)株在气管和肺组织的复制能力明显强于A/Chicken/Guangdong/SS/94(H9N2)AIV株。3株H9N2亚型重排AIVs的动物试验发现HA和NA基因对H9N2亚型AIV在呼吸道的复制特性起主要作用。内部基因对H9N2亚型AIV在呼吸道的复制也有一定的作用。结果表明1994年中国首次分离到的H9N2亚型AIV经过4年的宿主适应和基因进化,加强了其在呼吸系统的复制能力,奠定了气溶胶传播的基础。To explore the replicating feature of H9 avian influenza virus （AIV） in chickens, A/ Chicken/Guangdong/SS/94（H9N2） （SS） strain,which representing the earlier strain isolated in chickens in Guangdong province in 1994, and A/Chicken/Shanghai/F/98（H9N2） （F） strain, which was isolated in Shanghai during 1998 pandemic period, were selected as represent strains in this research. Three recombinants were constructed based on 6 genes from F strain and HA,NA genes from SS strain by reverse genetic technique. RF7/SSHA virus was composed of the HA gene from SS strain and the remaining seven genes from F strain, RF7/SSNA virus was composed of the NA gene derived from SS strain and the remaining seven genes from F strain, and RF6/ SSHA/SSNA virus was composed of the HA and NA genes of SS strain and the remaining 6 genes from F strain. Then the replicating property of the five H9N2 viruses were compared in SPF chickens. Groups of three 4-week old SPF chickens were inoculated with the AIVs by oral, tracheal and nasal routes. Virus replication was monitored 3 days after inoculation. Virus load in terms of ELDs0/EIDs0 in trachea, lung and intestine were assayed 3 days after inoculation. The results showed that the replicating property of H9N2 subtype AIVs in chickens are determined by HA and NA genes mainly, and also related to internal genes. After 4 year＇s adaption and gene evolution, F strain isolated in 1998 have stronger replication ability than earlier AIV strain in respiratory system, which lay the foundation of aerosol infection.

关 键 词：禽流感病毒 H9N2亚型 复制特性 反向遗传技术 HA基因 NA基因 

分 类 号：S852.659.5[农业科学—基础兽医学] Q939.4[农业科学—兽医学]