淫羊藿多糖的硫酸化修饰及其对IBDV感染细胞的影响  

Sulfated Modification of Epimedium Polysaccharide and Effects of the Modifiers on Cellular Infectivity of IBDV

作　　者：卢宇[1] 胡元亮[1] 黄小燕[1] 王德云[1] 王君敏[1] 徐玉凤[1] 赵晓娜[1] 

机构地区：[1]南京农业大学中兽医学研究室,南京210095

出　　处：《畜牧兽医学报》2008年第2期251-256,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30571360);江苏省普通高校研究生科研创新计划资助项目(2006)

摘　　要：按正交试验设定氯磺酸-吡啶法的9种修饰条件,对淫羊藿多糖(EPS)进行硫酸化修饰,得到9种硫酸化淫羊藿多糖(sEPS),用MTT法比较了9种sEPS抵抗鸡传染性法氏囊病毒(IBDV)感染鸡胚成纤维细胞(CEF)的作用。结果表明,硫酸化修饰后sEPS较未修饰的EPS显著提高了CEF抵抗IBDV感染的作用,以sEPS2和sEPS5的效果较好,且与sEPS的硫酸基取代度和糖含量有一定的相关性,反应温度80℃、氯磺酸与吡啶的配比1∶8、反应2h为最佳修饰条件。Nine modification conditions were designed to sulfate epimedium polysaccharide （EPS） by chlorsulfonic acid-pyridine method as orthogonal test, and nine sulfated EPS （sEPS） were ob- tained. The effects of the nine sEPS on IBDV infecting chick embryo fibroblast （CEF） was compared by MTT assay. The results showed that modified sEPS had significant enhanced resisting effect of CEF on IBDV infection in comparison with non-sulfated EPS especially sEPS2 and sEPS5 presented better effects. The enhanced resisting effects of sEPS have certain relationships with the degree of sulfation （DS） and carbohydrate content. The optimum modification conditions were the reaction temperature of 80℃, the ratio of chlosulfonic acid to pyridine of 1 ： 8 and the reaction time of 2 h.

关 键 词：淫羊藿多糖 硫酸化修饰 鸡传染性法氏囊病毒 感染细胞 

分 类 号：S853.74[农业科学—临床兽医学]