Cx40 mRNA expression in crista terminalis and left atrium of patients with rheumatic heart disease associated chronic atrial fibrillation  

作　　者：Zhao Feng Li Li Xu Zhiyun Huang Xing Zhou Yong 

机构地区：[1]Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China [2]Department of Cardiology, No. 411 Hospital of PLA, Shanghai 200081, China

出　　处：《Journal of Medical Colleges of PLA(China)》2008年第1期26-30,共5页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(30070749).

摘　　要：Objectives： To explore possible mechanisms of connexin40 （Cx40） remodeling by detecting Cx40 mRNA expression of the crista terminalis and left atrium （LA） in patients with rheumatic heart disease （RHD） associated chronic atrial fibrillation （AF）. Methods： Twenty patients were enrolled in this study, who underwent surgical operation for RHD-associated mitral disease, including l0 with sinus rhythms （rhythm group） and l0 with AF （AF group）. Another 6 patients with non-RHD sinus rhythms were divided into the control group. A small amount of myocardial tissue was cut from the crista terminalis and the LA posterior wall during the valvular replacement operation. Cx40 mRNA expression was assayed by real-time fluorescent quantitation polymerase chain reaction （RT-PCR）. Results： There was no significant difference in Cx40 mRNA expression in the crista terminalis and LA posterior wall between the 3 groups, and there was no significant difference in Cx40 mRNA expression between the crista terminalis and LA within each group. Conclusion： Based on the finding in previous studies that there existed evident remodeling of atrial Cx40 protein in patients with chronic RHD, the results of the present study suggest that the mechanism of Cx40 remodeling probably lies in the post transcriptional level.ObjectiveTo 探索由检测改变冠终端的 mRNA 表示是并且与联系的风湿性心脏病( RHD )在病人离开中庭( LA )的 Cx40 的 connexin40 ( Cx40 )的可能的机制在伊朗的银币长期纤维性颤动( AF ) .MethodsTwenty 病人在这研究被注册，谁与室性节律(节奏组)为 联系RHD 的二尖瓣病经历了外科的操作，包括 10 并且 10 与 AF ( AF 组)。另一有 non-RHD 室性节律的 6 个病人被划分成控制组。心肌的组织的小数量从冠终端被割并且 LA 以后的墙在瓣膜的代替操作期间。 Cx40 mRNA 表示是由聚合酶链反应( RT-PCR ) .ResultsThere 是的即时荧光灯的 quantitation 的 assayed 在在冠终端的 Cx40 mRNA 表示的有效差量都不是，在 3 之间的 LA 以后的墙组织，并且在在以前的研究发现在那里存在上的每 group.ConclusionsBased 以内在在冠终端之间的 mRNA 表示是的 Cx40 和 LA 没有有效差量明显的改变在在有长期的 RHD 的病人的伊朗的银币 Cx40 蛋白质，现在的学习的结果建议改变的 Cx40 的机制可能躺在帖子 transcriptional 水平。

关 键 词：CARDIOLOGY Rheumatic heart disease Connexin40 Atrial fibrillation Real-time fluorescent quantitation polymerase chain reaction 

分 类 号：R541.2[医药卫生—心血管疾病]