重组人甲状旁腺素相关蛋白PTHrP1-86对骨质疏松大鼠治疗作用的实验研究  

Study of treating effect to osteoporosis of recombinant human parathyroid hormone related protein 1-86

作　　者：郭刚[1] 刘新宇[1] 梁东春[1] 张瑞[1] 张镜宇[1] 

机构地区：[1]天津医科大学代谢病医院

出　　处：《中国骨质疏松杂志》2008年第1期13-15,共3页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(39970348);天津市自然科学基金重点资助项目(993804211)

摘　　要：目的探讨重组人甲状旁腺素相关蛋白PTHrP1-86对骨质疏松大鼠的骨同化作用。方法用摘除大鼠双侧卵巢的方式制备骨质疏松模型(OVX),去势手术16周后,测定大鼠腰椎骨密度,确认动物模型造模成功。实验动物分为8个组,给药后测定骨密度、骨钙素、骨形态计量学、骨生物力学等指标,初步评价重组人甲状旁腺素相关蛋白PTHrP1-86对骨质疏松大鼠的骨同化作用。结果重组人甲状旁腺素相关蛋白PTHrP1-86可使骨转换加快,促进新骨生成,使骨的力学性能提高,抗弯曲强度提高,抗变形能力增强,韧性增高,脆性降低,不易发生断裂。结论PTHrP1-86能有效提高骨的抗骨折能力,对治疗和预防骨质疏松有一定疗效。同时证明该动物实验方法行之有效,可以很好地测定PTHrP对骨质疏松的治疗作用,并可以此为依据开始一期临床实验。Objective Mensurate the osseous assimilation of human PTHrP1-86 with animal test. Methods Produce the model of emasculated animals and send medicine. Mensurate biomechanical, bone metrology and biochemical indexes which reflect the bone reconstruction to understand the influence of PTHrP on the osteoblast interaction, and to confirm the bioactivity of different fragments. Results The experimental results confirm that PTHrP1-86 can accelerate the bone conversion and the formation of new bone, improve the mechanical ability, the bending resistance, metaphosis resistance, and the tenacity, and reduce the frangibility, leading to harder breakage, demonstrating that PTHrP1-86 can efficiently improve the fraction resistance, and can treat and prevent osteoporosis. Conclusions PTHrP1-86 can efficiently improve the fraction resistance, and can treat and prevent osteoporosis.

关 键 词：人甲状旁腺素相关蛋白 骨同化作用 骨密度 骨形态计量学 骨生物力学 

分 类 号：R31[医药卫生—基础医学]