去势大鼠骨髓细胞IL-6基因表达及骨组织学变化观察  

Observation of the expression of interleukin-6 gene in bone of ovariectomized rats and the change of bone tissue

作　　者：周胜虎[1] 刘兴炎[2] 白孟海[2] 葛宝丰[2] 薛云[2] 

机构地区：[1]兰州大学医学院,730050 [2]兰州军区兰州总医院骨科

出　　处：《中国骨质疏松杂志》2008年第1期28-30,共3页Chinese Journal of Osteoporosis

基　　金：全军科研基金课题资助项目(01Z2008)

摘　　要：目的观察去势大鼠骨髓细胞白细胞介素-6基因表达和骨组织学的变化。方法健康3月龄雌性SD大鼠48只,24只作为假手术对照组,24只手术切除双侧卵巢。分别于去卵巢后4、6、8、12周取去势组和对照组各6只大鼠骨髓细胞提取RNA(左侧股骨)。PCR方法检测IL-6基因表达和骨组织学(右侧股骨)观察。结果术后4周,实验组骨髓细胞IL-6基因均呈弱表达,6周呈强表达,8和12周表达水平较峰值下降,但仍高于对照组。去势后对照组各时间段IL-6基因表达水平未见变化。术后4、6和8周实验组骨组织学未见明显变化;12周骨髓腔扩大,骨皮质变薄,骨小梁变细,见断裂现象。结论绝经后骨质疏松的发生与IL-6基因水平增强有一定的关系。Objective To observe the expression of Interleukin-6 gene in bone of ovariectomized rats and the change of bone tissue. Methods Fourty-eight SD rats were randomly divided into 2 groups： Shame-operated control group（N） and ovariectomized group （OVX）. At the end of week 4,6,8 and 12 after ovariectomy; RNA were collected from the rat femur bone marrow cells （left femur） of 12 rats, 6 from OVX group and 6 from N group. The expression of IL-6 gene was determined by PCR and the change of bone tissue （right femur）. Results Four weeks after the surgery, IL-6 gene expression of bone marrow cells declined in experiment groups, but it peaked at 6th week, In 8th-12th weeks after ovariectomy, the gene expression declined, but still significantly higher than in control group. IL-6 gene expression did not change in different times in OVX. In 4^th , 6^th , 8^th week after the surgery, the change of bone tissue was not obvious in experiment groups, at 12^th week, changes of bone tissue section were observed ： thinner cortical plate and fracture of bone trabecula. Conclusions Postmenopausal osteoporosis may be related to the increase of IL-6 gene expression.

关 键 词：去势大鼠 白细胞介素-6 基因表达 骨质疏松 骨组织学 

分 类 号：R31[医药卫生—基础医学]