2型糖尿病雌性大鼠骨代谢的特点  

Changes of bone mineral density and bone biomechanics in type 2 diabetic model of female rats

作　　者：刘亚平[1] 季虹[2] 荣海钦[2] 张丽萍[2] 赵新波[2] 邱占军[2] 

机构地区：[1]山东省医学科学院在读硕士研究生,济南250062 [2]山东省内分泌与代谢病研究所

出　　处：《中国骨质疏松杂志》2008年第1期35-38,共4页Chinese Journal of Osteoporosis

摘　　要：目的研究2型糖尿病雌性大鼠骨密度和骨生物力学等指标的变化及各指标间的相互关系。方法采用高糖高脂饮食加腹腔注射小剂量链脲佐菌素诱导建立2型糖尿病大鼠模型。20周后处死大鼠,测定股骨和腰椎骨密度(BMD)、骨灰干重比、骨生物力学以及骨代谢相关指标[血清Ca、骨钙素(BGP)、抗酒石酸酸性磷酸酶(TRAP)、尿Ca/肌酐比值、尿羟脯氨酸/肌酐比值],此外还观察了空腹血糖(FPG)、GHbA1c、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、血清胰岛素(FINS)、胰岛素样生长因子(IGF)-Ⅰ等变化。结果与正常组相比,(1)糖尿病大鼠腰椎和股骨BMD、灰干重比、生物力学指标显著降低;(2)FPG、FINS及HOMA-IR均显著升高,说明存在胰岛素抵抗;(3)血清TRAP、TG、TC、LDL-C、GHbA1c升高,血清BGP、IGF-Ⅰ、HDL-C降低,差异均有显著性;(4)BGP与股骨BMD成显著正相关。结论高糖高脂饲料联合小剂量链脲佐菌素注射,可成功建立2型糖尿病雌性大鼠模型。该模型大鼠具有高血糖、脂质代谢紊乱和胰岛素抵抗特点。2型糖尿病大鼠骨吸收加快而骨形成不足,导致其骨量下降和骨生物力学特性改变。Objective To study the changes on bone metabolic characteristics, bone mineral density （BMD） and bone biomechanics in type 2 diabetic model of female rats, and investigate the relationships between these indexes. Methods Type 2 diabetic model of rats was set up by feeding high-glucose-high-fat diet and by injecting intraperitoneally with a low-dose streptozotocin （STZ）. All rats were sacrificed after 20 weeks. The BMD of femora and lumbar vertebra, the ratio of ash weight to dry weight, the biomechanical parameters and the bone metabolic related indexes, such as serum Ca, BGP, TRAP, urinary calcium/creatinine（Ca/Cr）, urinary hydroxyproline/ creatinine（HYP/Cr）, and fasting blood glucose, GHbA1c, TG, TC, HDL-C,LDL-C,fasting serum insulin, IGF-Ⅰwere measured. Results Compared with normal control group, the BMD of femora and lumbar vertebra, the ratio of ash weight to dry weight and the bone biomechanical indexes of type 2 diabetic rats decreased rapidly. Insulin resistance was induced in rats of type 2 diabetes mellitus as the level of FPG, FINS, HOMA-IR markedly increased. Serum concentrations of TRAP, TG, TC, LDL-C, GHbA1c markedly increased, and BGP, IGF- Ⅰ , HDL-C decreased. There were even significant differences. BMD of femora showed a positive correlation with BGP strongly. Conclusions The rat model of type 2 diabetes mellitus which is similar to human type 2 diabetes mellitus is induced by feeding high-glucose-high-fat diet and by intraperitoneal injection with a low-dose STZ. This rat model is characterized by moderate hyperglycemia, lipid disorder and insulin resistance. The Bone resorption was accelerated and the formation decreased in this type 2 diabetic model rats, which consequently resulted in the descending bone mass and the changes of the bone biomechanical properties.

关 键 词：2型糖尿病 大鼠 骨组织 骨密度 生物力学 

分 类 号：R31[医药卫生—基础医学]