茄尼醇转化高产辅酶Q10菌株的分离鉴定与发酵条件的研究  

Isolation,Characterization and Fermentation Condition of Coenzyme Q_(10) Producing Strain with Solanesol as Precursor

作　　者：柳华贵[1] 方建军[1] 金凌[1] 钟卫鸿[1] 叶紫娟 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310032 [2]杭州利群环保纸业有限公司,杭州310018

出　　处：《微生物学报》2008年第2期157-163,共7页Acta Microbiologica Sinica

基　　金：浙江省科技厅重点项目(2007C23035);浙江省生物化工重中之重学科开放基金(200601)~~

摘　　要：采用以异戊二烯为唯一碳源的选择性平板筛选模型,从钱塘江沿岸杭州市九堡段土壤中新筛选到一株产辅酶Q_(10)的细菌菌株E03,经形态、生理生化、Biolog碳源利用试验和16S rDNA序列分析,确定E03属于鞘氨醇属(Sphingomonas sp.),命名为Sphingomonas sp.ZUTE03。摇瓶试验确定了该菌发酵生产辅酶Q_(10)的最佳碳源为葡萄糖15 g/L,氮源为硫酸铵10 g/L,初始pH8.0,发酵温度25℃,并考察了该菌转化茄尼醇产辅酶Q_(10)的发酵工艺,以合适溶剂为溶解体系,于发酵培养基中摇床培养12 h后,加入终浓度为0.75 g/L的茄尼醇粗品,转化12 h,辅酶Q_(10)产值可达96.88 mg/L。A bacterium capable of producing Coenzyme Q10 （CoQ10, was isolated from soil. Based on analysis of the 16S rDNA sequence, traditional physiological characteristics, and Biolog-GN, the strain was belonging to the genus Sphingomonas and named as Sphingomonas sp. ZUTEO3. The optimum fermentation condition of CoQ10 production was as following： glucose 15 g/L, （NH4）2SO4 10 g/L, original pH 8.0 and at 25℃ The addition of solanesol could improve CoQ10 production. The optimal condition for the bioconservation from solanesol to CoQ10was as following： adding 0.75 g/L of raw solanesol after the first 12 h fermentation and continued for another 12 h fermentation. The maximal yield of CoQ10 reached 96.88 mg/L.

关 键 词：辅酶Q10 16S rDNA 鞘氨醇菌 发酵 

分 类 号：Q93[生物学—微生物学]