酵母单杂交系统在植物基因工程研究中的应用  

Application of yeast one hybrid system in plant gene engineering

作　　者：王琪[1] 朱延明[1] 王冬冬[1] 

机构地区：[1]东北农业大学生命科学学院

出　　处：《北京林业大学学报》2008年第1期141-147,共7页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30570990);黑龙江省科技厅重点攻关项目(GB05B104)。

摘　　要：酵母单杂交系统是由酵母双杂交系统衍生来的研究DNA与蛋白质之间相互作用的新型系统。该文系统阐述了单杂交系统的基本原理和技术路线,详细综述了其在植物基因工程各个领域的应用研究进展,即克隆抗渗透胁迫类转录因子基因,确定已知DNA与蛋白质之间的相互作用,定位已证实的具有相互作用的DNA结合结构域以及验证转录激活作用;并分析了当前该系统在植物基因工程研究中存在的问题,进而结合自己的研究对解决问题的途径进行了探讨。The yeast one hybrid system is the new type system deriving from yeast two hybrid system to study the interactions between DNA and protein. This paper briefly introduces the principle and technical line of this system, and particularly summarizes the progress of this system in plant genetic engineering field, such as cloning the transcription factor genes of osmosis resistance type, of embryogenesis type, and of disease resistance type; confirming the interaction between known DNA and protein; making sure the localization of the confirmed DNA binding domain ; moreover validating the transcriptional activation activity. Furthermore, the authors analyzed the problems existing in this system on the research of plant genetic engineering, as well as forecasted the development prospect according to the research.

关 键 词：酵母单杂交 植物基因工程 转录因子 转录激活结构域 DNA结合结构域 

分 类 号：Q943.2[生物学—植物学]