华支睾吸虫多药物／代谢产物外排蛋白样基因的识别和生物信息学分析  

Recognization and bioinformatics analysis of Clonorchis sinensis small multidrug/metabolites efflux protein-like gene

作　　者：胡旭初[1] 赵宇[1] 黄灿[1] 刘玲[1] 余新炳[1] 徐劲[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,“热带病防治研究”教育部重点实验室,广州510080

出　　处：《中国人兽共患病学报》2008年第2期104-108,共5页Chinese Journal of Zoonoses

基　　金：“863”专题(2006AA02Z422);广东省重大科技专项(2004A30801004);广东省自然科学基金重点项目(36627)资助

摘　　要：目的预测华支睾吸虫假想的多药物/代谢产物外排蛋白的结构及功能及其应用前景。方法利用多种生物信息学分析软件,从华支睾吸虫全长cDNA质粒文库中识别出多药物/代谢产物外排蛋白样基因,分析蛋白的拓扑学结构、生物学和免疫学功能特征,根据结构与功能预测其应用前景。结果该基因编码189个氨基酸;含有5段跨膜区,N端在胞内,C端在胞外,具有主要协助因子超家族的结构特征。与日本血吸虫同源蛋白(一致性为67%,相似性为80%)相比,该蛋白的4段跨膜区中含有保守的精氨酸和组氨酸残基。该蛋白含有多个磷酸化位点和T、B细胞表位,且线性B细胞表位均位于胞外区。结论所克隆的华支睾吸虫基因可能编码一个小蛋白型阴离子多药物/代谢物外排蛋白;该蛋白可能定位于华支睾吸虫皮层表膜,是一个潜在的诊断和疫苗候选抗原和药物靶标分子。A clone from Clonorchis sinensis full-length eDNA plasmid library was recognized as a putative small multidrug/metabolites efflux protein-like gene ,and the structural and functional characteristics of the putative protein were analyzed by on-line bioinformatics tools provided by NCBI （http：//www. ncbi. nlm. nih. gov/, ExPaSy （http：//www. ncbi. nlm. nih. gov/）, and CBS Prediction Servers （http,//www. cbs. dtu. dk/services/） and Vector NTI suite 8.0 software package,and its application was predicted on the basis of the bioinformatics. It was found that gene contained a complete cds encoding 189 amino acids containing 5 transmembrane regions with the N terminus inside of the cytoplasm membrane and C terminus outside. It belong to the subfamily of MFS-1 of the Major Facility Superfamily with the property of small multidrug/metabolites efflux protein, which was characterized by 4 or 5 transmembrane regions. Comparing with the homologue of Schistosoma japonicum （with identity of 67 % and similarity of 80 %）, each of four of five transmembrane regions contains one or two conserved basic amino acid residue arginine or histidine and there are several phosphorylation sites and linear T cell and B cell epitopes mainly located in the outside of membrane. These suggest the protein maybe locate in the membrane of tegument of C. sinensis and is involved in the pump-out of anionic drugs or deleterious metabolites, and is a potential candidate of vaccine and immunodiagnosis for clonorchiasis.

关 键 词：华支睾吸虫 小蛋白型多药物/代谢物外排蛋白 耐药性 诊断 疫苗 

分 类 号：R383.2[医药卫生—医学寄生虫学]