蒸汽灭菌对聚醚酯材料及其血管细胞相容性的影响  

Effect of Steam Sterilization on Poly(ether-ester)s Copolymer and Its Vascular Cell Compatibility

作　　者：王玮[1] 奚廷斐[2] 陈亮[2] 张爱英[1] 王连才[1] 

机构地区：[1]北京理工大学材料学院,北京100081 [2]中国药品生物制品检定所,北京100050

出　　处：《生物医学工程学杂志》2008年第1期117-121,共5页Journal of Biomedical Engineering

基　　金：国家“十五”863计划资助(2001AA216071)

摘　　要：探讨了蒸汽灭菌对聚乙二醇对苯二甲酸酯/聚对苯二甲酸丁二醇酯多嵌段共聚物(PEGT/PBT)性能的改变而导致对血管细胞相容性的影响。血管细胞能在紫外辐照灭菌的PEGT/PBT膜片上较好地黏附生长,而在蒸汽灭菌的膜片表面几乎无法黏附生长。使用差示扫描量热分析、静态接触角、光电子能谱、表面羧基测定、核磁共振和扫描电镜等分析测试方法对灭菌前后膜片的本体性能和表面性能进行表征。结果表明,蒸汽灭菌没有改变材料表面形貌和材料的组成。但是在蒸汽灭菌过程中PEGT/PBT链段发生重新取向,亲水性软段PEGT和材料的端羧基在表面富集,使得材料表面亲水性增加、硬段的结晶度有所增加。可能由于端羧基和表面聚乙二醇增多导致蛋白在材料表面的吸附减少,而致使血管细胞无法在蒸汽灭菌的膜片上黏附生长。The aim of this research was to evaluate the influence of steam sterilization on poly（ethylene glycol-terephthalate）and poly（buwlene terephthalate） copolymer （PEGT/PBT） and its vascular cells compatibility, which was used as the scaffolds in vascular tissue engineering. Endothelial cells, smooth muscle cells and fibroblasts were cultured separately on the films after steam sterilization and after ultraviolet sterilization. These cells can grow well on the films after ultraviolet sterilization, while they can hardly adhere on steam sterilized films. Differential scanning calorimetry, static contact angle, X-ray photoelectron spectroscopy, surface carboxyl density quantity, H-nuclear magnetic resonance and scanning electronic microe, cope were employed to characterize the properties of poly（ether-esters） films before and after sterilization. These results showed that steam sterilization had little effect on the surface morphology and on the constitution of the copolymer, but the copolymer segments were redistributed during steam sterilization. The hydrophilic poly（ethylene glycol） （PEG） and the end carboxyl groups transferred from the bulk and enriched on the surface and the degree of crystallinity of hard segments increased slightly. Both the end carboxyl and PEG enriched on the surface can hinder the protein adhesion on the surface; so, lacking in receptor, the vascular cells cannot adhere on the films surfaces.

关 键 词：聚乙二醇对苯二甲酸酯/聚对苯二甲酸丁二醇酯 蒸汽灭菌 紫外辐照灭菌 血管细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]