慢性束缚应激对移植S180肿瘤小鼠肿瘤生长的影响  

The effect of chronic restraint stress on tumor growth in mice transplanted S180 tumor

作　　者：杨宁波[1] 贺丹军[1] 吴爱勤[1] 李洁[1] 

机构地区：[1]江苏省人民医院临床心理科,南京210029

出　　处：《中国行为医学科学》2008年第2期120-122,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的观察慢性束缚应激下荷瘤小鼠肿瘤的生长情况及病理改变。方法40只昆明小鼠建立慢性束缚应激模型，随机分为单纯肿瘤组（ST组）和肿瘤＋应激组（T＋S组）。应激后处死小鼠，剥取肿瘤称重、制作肿瘤组织HE片，在显微镜下进行组织形态学观察并显微拍照。结果T＋S组瘤重[（1．69±0．33）g]明显较ST组重[（1．02±0．57）g]，P〈0．01；T＋S组小鼠瘤细胞在异型性、瘤周及瘤内炎症三方面与ST组比较，差异有显著性，分别为[ST组（1．18±0．62）vsT＋S组（3．00±0．00），P〈0．05；ST组（2．02±0．36）vsT＋S组（0．78±0．16），P〈0．01；ST组（1．89±0．17）VST＋S组（1．07±0．45），P〈0．05]。结论慢性心理应激促进了肿瘤的发生、生长并使肿瘤的恶性程度增加。Objective To observe growth circumstance and pathologic change of transplanted S180 tumor following chronic restraint stress. Methods 40 Kunming mice were randomly divided into simple tumor group（ n = 20 ） and stress add tumor group （ n = 20 ）. Restraint device were 50-ml polypropylene conical tubes, and restraint stress was imposed 8h per night . After 14 days mice were killed and tumor were taken. Then weigh the tumor before paraffin section of HE were made. Then pathologic change of tumor and photomicrographs were observed. Results The average tumor weight of T ＋ S group are obviously heavier than that of ST group [ （ 1. 6918 ±0. 3300） g vs （ 1. 0224 ±0. 5680） g, P〈0.01 ] ;there are significant difference between T ＋ S and ST group at aspects of beteromorphy and phlogosis outside or inside of the tumor , which respectively is [ （3.00 ±0.00） vs （1. 18 ± 0.62） , P〈0.05;（0.78 ±0.16） vs （2.02±0.36）P〈0.01;（1.07 ±0.45） vs （1.89±0.17）,P〈0.05]. Conclusion Chronic restraint stress can to promote the occurrence and growth of the tumor,and make the tumor more malignant.

关 键 词：束缚应激 移植肿瘤 病理形态 

分 类 号：R285.5[医药卫生—中药学] R730.2[医药卫生—中医学]