芪术合剂对肺纤维化大鼠TGF-β1表达及肺组织形态的影响  

Effect on Expression of TGF-β1 and Morphous of Lung Tissue in Pulmonary Fibrosis Rats Interfered with QiZhuHeJi

作　　者：刘恩顺[1] 廉富[1] 孙增涛[1] 封继宏[2] 李小娟[1] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学,天津300193

出　　处：《辽宁中医杂志》2008年第2期301-303,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：天津市自然科学基金资助项目(023611811)

摘　　要：目的:观察芪术合剂对博莱霉素致大鼠肺间质纤维化的干预作用及其机制。方法:将60只健康SD大鼠随机分为正常组、模型组、芪术合剂组和强的松组。经气管滴入博莱霉素复制肺纤维化大鼠模型,并分组予以治疗,于治疗3、7、21天分批处死各组大鼠,每次每组6只。观察肺组织TGF-β1的表达和病理变化。结果:模型组在不同时段TGF-β1表达较正常对照组明显增高,芪术合剂组与同期模型组比较明显降低,芪术合剂组和强的松组经干预后肺泡炎和肺纤维化有所减轻。结论:中药芪术合剂具有显著抑制博莱霉素致大鼠肺纤维化的作用,抑制TGF-β1的表达是其可能的机制之一。Objective ： To observe the effect and mechanism on bleomycin - induced pulmonary fibrosis in rats interfered with QiZhuHeJi（ QZHJ）. Method： 60 SD rats were divided into four groups randomly： normal control group, model group, QZHJ treated group, and hydrocortisone treated group. The rats were duplicated pulmonary fibrosis model by intratraeheal instillating of bleomycin, and each group was given corresponding drugs respectively. The experimental rats were sacrificed in batch on the 3rd,7th and 21st day for observing TGF- β1 expression and pathological change of lung tissue. Result ：It was showed that TGF - β1 expression in different stage in model group increased obviously and showed significant difference in comparison with normal group. TGF - β1 expression in QZHJ treated group decreased significantly compare with hydrocortisone treated group in the same stage. And there are significant difference. After intervention, airsacculitis and fibrosis were improved in QZHJ treated group and hydrocortisone treated group. Conclusion ： QZHJ has a notable effect in restrain rats pulmonary fibrosis induced by bleomycin and inhibition of TGF - β1 expression is one of its possible mechanisms.

关 键 词：肺纤维化 芪术合剂 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]