细辛挥发油对颅痛定的裸鼠体外促透的作用  

In vitro effect of volatile oil of Herba Asari on the permeation of Rotundine through nude mouse skin

作　　者：程欣[1] 马云淑[1] 阎红[1] 张晓雷[1] 李艳杰[1] 

机构地区：[1]云南中医学院中药学院,云南昆明650200

出　　处：《华西药学杂志》2008年第1期4-6,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金项目(批准号:30560187)

摘　　要：目的考察细辛挥发油对颅痛定的体外促透皮作用,筛选中药透皮促进剂。方法采用改良的Franz扩散池进行体外透皮实验,以裸鼠皮肤为渗透屏障、5%氮酮为对照,观察5%细辛挥发油对颅痛定的促透皮作用。采用HPLC法测定颅痛定的含量,计算24 h平均累积渗透量与12 h平均透皮速率常数。结果15 mg.m l-1颅痛定加入5%细辛挥发油或5%氮酮后,24 h平均累积渗透量分别为5.723×103、4.384×103μg.cm-2;12 h平均透皮速率常数分别为284.29、223.18μg.cm-2.h-1;增渗倍数分别为37.09、29.12。与阴性对照组相比,透皮速率常数在数值上差异有非常显著性意义。结论5%细辛挥发油对颅痛定有较强的促透皮作用,其效果优于5%氮酮。OBJECTIVE To investigate the enhancing effects of volatile oil of Herba Asari on the permeation of Rotundine through in vitro nude mouse skin and screen permeation enhancer from Traditional Chinese Medicine. METHODS A test of enhancing effect of 5% volatile oil of Herba Asari on the permeation of Rotundine was conducted by an improved method of Franz diffusion cells through nude mouse skin and compared with 5% azone. The content of Rotundine was measured by HPLC and the average cumulative doses for 24 hours and rate constants for 12 hours of skin permeation of Rotundine were calculated. RESULTS After adding 5% volatile oil of Herba Asari or 5 % azone, the average cumulative doses of skin permeation of Rotundine （ 15 mg· ml^- 1 ） for 24 hours were 5722. 78 and 4384. 31 μg· cm^ - 2, respectively ; the rate constants of skin permeation for 12 hours were 284. 29 and 223. 18 μg· cm^ - 2· h ^- 1, respectively; and the multiple of anatonosis were 37. 0934 and 29. 1189 respectively. Compared with negative control, the contrast of rate constants of skin permeation was obviously significance. CONCLUSION 5% volatile oil of Herba Asari possesed the effects of the permeation enhancement on Rotundine in vitro, and exceeded 5% azone.

关 键 词：颅痛定 细辛油 氮酮 促透皮 体外 HPLC 

分 类 号：R96[医药卫生—药理学]