白花丹醌对人乳腺癌细胞mda-mb-231的体外效应  

Effects of plumbagin on the human breast cancer cell mda-mb-231 in vitro

作　　者：刘超[1] 刘圆[1] 颜晓燕[2] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041 [2]成都医学院药学系药理教研室,四川成都610083

出　　处：《华西药学杂志》2008年第1期42-44,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省青年科技基金(07ZQ026-011);四川省应用基础项目(2006);四川省科技攻关项目(2006);西南民族大学教学改革项目(No.2005)

摘　　要：目的探讨白花丹醌的体外抗肿瘤活性。方法采用MTT染色法和集落形成法探讨白花丹醌对乳腺癌细胞株mda-mb-231的体外抑制生长作用。同时,以人胚肺成纤维细胞株MRC-05为对照,初步探讨了白花丹醌对正常细胞的细胞毒作用。结果白花丹醌对mda-mb-231有较好的抑制生长作用,IC50=0.263 g.L-1,对乳腺癌细胞克隆原形成的IC50=5.13×10-3g.L-1;而对人胚肺成纤维细胞MRC-05的细胞毒作用弱,IC50=5.13×10-3g.L-1。结论白花丹醌的细胞毒作用可能具有一定的选择性。OBJECTIVE To observe the antitumor activity of plumbagin in vitro. METHODS Cancer ceils were cultured in vitro, MTI＇ method and colony -forming method were used to detect the inhibitory effects on mda - mb -231. On the other hand, human embryonic lung fibroblast MRC - 05 was used as control to detect the cytotoxic effect of plumbagin on normal cell. RESULTS Plumbagin has significant antitumor activity on mda- mb- 231, the IC50 was 0. 263 g· L^-1 by MTT and 5. 13 × 10-3 g· L^-1by colony -forming method . CONCLUSION Plumbagin has significant antitumor activity on mda - mb - 231. The selective cytotoxic effect of the plumbagin is worth studying further.

关 键 词：白花丹醌 人乳腺癌细胞 体外试验 

分 类 号：R96[医药卫生—药理学]