GC测定荆芥及荆芥油中的薄荷酮和胡薄荷酮  

Determination of menthone and pulegone in Schizonepeta tenuifolia and Schizonepeta oil by GC

作　　者：唐杨[1] 颜挺[1] 晁若冰[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2008年第1期102-104,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的测定荆芥及荆芥油中的薄荷酮和胡薄荷酮。方法采用GC法。用PEG-20M石英毛细管柱（30m×0．53mm×1μm），FID检测器，氮气为载气，柱温140℃，气化室和检测器温度均为250℃，不分流进样。以萘为内标。结果薄荷酮的线性范围为0．2～2．5mg·ml^-1（r=0．9999），胡薄荷酮的线性范围为0．2～2．5mg·ml^-1（r=0．9992）；薄荷酮和胡薄荷酮的平均回收率（n=9）分别为99．0％、99．6％。结论所建方法简便、准确，可同时测定荆芥以及荆芥油中的薄荷酮和胡薄荷酮。OBJECTIVE To establish a method for determination of menthone and pulegone in Schizonepeta tenuifolia and Schizonep etaoil. METHODS The contents of menthone and pulegone were determined by GC with PEG - 20M fused capillary column （30 m × 0. 53 mm, 1 μm） and FID detector, nitrogen as the carrier gas. Both injector and detector temperatures were set at 250℃, and the col- umn temperature was 140℃. The method was employed with naphthalene as the internal standard. RESULTS The linear ranges were 0. 2 - 2; 5 mg· ml^-1 （ r = 0. 9999） for menthone and 0. 2 - 2.5 mg· ml^-1 （ r = 0. 9992 ） for pulegone, the mean recoveries （ n = 9 ） of menthone and pulygone were 99.0% and 99.6% , respectively. CONCLUSION The method is simple and accurate, which can be applied to the determination of menthone and pulegone in Schizonepeta Tenuifolia and Schizonepetaeoil.

关 键 词：气相色谱法 荆芥 荆芥油 薄荷酮 胡薄荷酮 含量测定 

分 类 号：R917[医药卫生—药物分析学]