淇河鲫生长激素(growth hormone)全长cDNA的克隆与序列分析  

Cloning and sequencing of full length growth hormone cDNA from Carassius auratus gibelio var

作　　者：高春生[1] 范光丽[2] 杨国宇[1] 裴淑丽[1] 王艳玲[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]西北农林科技大学动科学院,陕西杨凌712100

出　　处：《分子科学学报》2008年第1期21-26,共6页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(39470827);河南省杰出青年基金资助项目(970203011)

摘　　要：用RT-PCR法和3′、5′RACE(Rapid amplification of cDNAends)法从淇河鲫脑垂体RNA克隆出生长激素(Growth hormone,GH)cDNA.此cDNA全长1191 nt(含Poly(A)14 nt),其5′端非编码区长55 nt、阅读框(Open readingframe,OAF)长633 nt,3′端非编码区长503 nt.其推导的GH由210个氨基酸组成,其中氮端前22个氨基酸为信号肽部分.氨基酸序列比较表明:淇河鲫与同目的鲫鱼、鲤鱼、团头鲂和斑马鱼的同源性分别为98.6%,96.2%,91.5%和88.6%;与不同目鱼的胡子鲇和鳗鲡的同源性分别为74.6%和49.5%;与哺乳类的家鼠和人等的同源性低于40%.The full length eDNA encoding growth hormone of a freshwater fish, Carassius auratus gibelio var, was cloned from pituitary RNA with RT-PCR, 3′and 5′RACE （rapid amplification of eDNA ends）. The GH cDNA, about 1 191 nt（nueleotide）long,consisted of a open reading frame with 633 nt long,5′ and 3′ untranslated regions with 55 nt and 503 nt long respectively, and a 14nt poly （A）tail. The DNA sequence analysis showed that there is a polyadenylation signal. The pregrowth hormone peptide of 210 AA deduced from CagvGH cDNA included a putative signal peptide（22 AA） locating in its N-terminal. Homological eomparision among CagvGH AA and other species growth hormones showed that the homogeneities were 98.6% ,96.2% ,91.5% ,88.6% ,74.6% ,and 49.5% compared with Carassius auratus, Cyprinius carpio, Megalobrama amblycephala, Danio refio, Clarias batrachus, and AnguiUa japonica,but under 40% compared with Mus musculus and Homo sapiens.

关 键 词：生长激素CDNA RT-PCR 淇河鲫 

分 类 号：Q785[生物学—分子生物学]