新基因TSC43在小鼠和人睾丸组织中的表达分析  

Character analysis of a novel testis-specific TSC43 gene in mouse and human testis

作　　者：余振东[1] 吴波[1] 唐爱发[1] 桂耀庭[1] 张立兵[1] 张键荣[1] 江智茂[1] 蔡志明[1] 

机构地区：[1]北京大学深圳医院,深圳518036

出　　处：《中国实验诊断学》2008年第2期143-146,共4页Chinese Journal of Laboratory Diagnosis

基　　金：广东省自然科学基金资助项目(7008952);国家自然科学基金资助项目(30700824)

摘　　要：目的筛选与精子发生相关的睾丸特异性新基因。方法将不同发育阶段的小鼠睾丸组织cDNA探针与Affymetrix全基因组芯片进行杂交,通过杂交信号的比较,筛选出差异表达的基因。用生物学信息软件对该基因进行生物学信息分析。RT-PCR分析该基因在小鼠不同发育阶段睾丸、小鼠不同组织及人不同组织中的表达。结果通过4、9、18、35、54日龄和6月龄小鼠睾丸的芯片信号比较筛选出一个差异表达基因(GenBank登录号:XM-156106),全长有1364 bp,含有1146 bp的完整ORF,编码一个有381个氨基酸、分子量为43.578 kDa的蛋白质,我们将其命名为TSC43。亚细胞定位预测显示TSC43基因可能在细胞核中表达。EBI功能域分析表示该基因可能参与了cAMP依赖的PK(Protein kinase)的锚定。RT-PCR分析表明TSC43基因特异性表达于小鼠睾丸组织中且具有时序性表达。TSC43蛋白在人的同源基因GenBank登录号为NM-152539,在381个氨基酸区域内有49%的同源性,人TSC43(hTSC43)基因特异性地表达于人睾丸组织中。结论TSC43基因的表达与小鼠精子发生的过程一致,在小鼠及人的睾丸组织中特异性表达,显示其可能在精子发生中起着重要作用。Objective To identify novel genes involved in spermatngenesis.Methods Testes cDNA samples from Balb/C mice of different postnatal days were performed with mouse whole genome Affymetrix Geneehip to semen novel testis-specific genes. The tissues distribution of the testis-specific genes in mice organs was identified by RT-PCR. The characteristics of selected genes were analyzed by various bioinformatie tools.Results A novel testis-specific gene （GenBank Accession No：XM_ 156106） with a differential expression in the developmental stages of testis was identified. The full length cDNA of 1364 bp contained an open reading frame of 1 146bp which encoded a putative protein of 381 amino acids,we named this gene TSC43. RT-PCR analysis revealed that TSC43 mRNA was exclusively expressed in mice and human testis. Cellular mapping prediction of TSC43 indicated that its protein would be expressed in nuclear and one putative domain was anchoring domain of cAMP-dependent type Ⅱ PK predicted at the protein site 332- 377. Conclusion The expression of TSC43 corresponds to the appearance of spermatid, indicating TSC43 may be involved in testis spermatngenesis.

关 键 词：基因芯片 克隆 TSC43基因 精子发生 RT-PCR 

分 类 号：R346[医药卫生—基础医学]