基于纳米材料、聚电解质制备高性能乙醇生物传感器  

Highly Sensitive Ethanol Biosensor based on Nano-materials and Polyelectrolytes

作　　者：李莎[1] 王艳艳[1] 赵紫霞[1] 秦霞[1] 王新胜[1] 吴宝艳[1] 陈强[1] 

机构地区：[1]南开大学生命科学学院生物活性材料教育部重点实验室,天津300071

出　　处：《分析化学》2008年第2期227-230,共4页Chinese Journal of Analytical Chemistry

基　　金：天津科技发展基金资助项目(No.06YFGNC03900)

摘　　要：以明胶包埋法作为乙醇氧化酶（AOX）的酶固定化方法，在比较多壁纳米碳管（MWCNTs）、纳米金、纳米氧化铁3种纳米材料的基础上，选择加入增效最明显的多壁纳米碳管提高灵敏度，在电极表面自组装聚丙烯胺盐酸盐／聚磺化乙烯硫酸盐（PAA／PVS）膜提高抗干扰性，制备出一种高灵敏度抗干扰性电流型乙醇生物传感器（Pt/（PAA／PVS）2PAA-MWCNTs／AOX）。本传感器灵敏度为1．04μA／（mmol／L），在2．5×10^-5～2．5×10^-3mol／L浓度范围内呈良好线性关系，线性方程为：，I（μA）=0．2199＋1．0400C（乙醇，mmol／L），r=0．9939：检出限为2．5×10^-5mol／L；RSD〈5％，稳定性好、抗干扰能力强。A novel amperometric ethanol biosensor with high sensibility and good anti-interference property has been developed. In this work, the gelatin embedding technique was used for the immobilization of alcohol oxidase （AOX） ; After comporing the effects of MWCNTs, gold nano-particles （GNp） and ironoxide nanoparticles （IONp） on the biosensor property, MWCNTs was selected to improve the sensibility of biosensor with the most marked effect. The anti-interference property of the biosensor was good because of the poly （ allylamine）/poly（ potassium vinyl sulphate）（PAA/PVS） nano-film which was introduced via layer-by-layer technique. This ethanol biosensor （pt/（ PAA/PVS）2PAA-MWCNTs/AOX） fabricated showed good linear correlation with the concentration of ethanol from 2. 5 × 10^-5 mol/L to 2. 5 × 10^-3 mol/L, with a linear regression equation of I（μA） =0. 2199 ＋ 1. 0400C（mmol/L） and a high response sensibility of 1.04μA./（mmmol/L）. The linear correlation coefficient and determination limit was 0. 9939 and 2. 5 × 10^-5 mol/L, respectively. The RSD was less than 5 %. Moreover, the stability and anti-interferential property were good.

关 键 词：乙醇 生物传感器 醇氧化酶 明胶包埋法 多壁纳米碳管 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]