基于担载贵金属纳米粒子聚苯二胺的生物传感器  

Biosensors Based on Polyphenylenediamine Loaded with Noble Metal Nanoparticles

作　　者：李新贵[1] 马小立[1] 黄美荣[1] 

机构地区：[1]同济大学材料学院先进土木工程材料教育部重点实验室材料化学研究所,上海200092

出　　处：《分析化学》2008年第2期253-258,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20774065)

摘　　要：系统论述了基于聚苯二胺的电流型生物传感器,如过氧化氢传感器、葡萄糖传感器和免疫传感器等的探测性能。指出聚苯二胺中的大量氨基/亚胺基及其膜的形态结构有助于加大贵金属纳米粒子的载入量,这种载有贵金属的聚苯二胺对酶的高效锚定作用以及贵金属纳米粒子的快速电子传输能力使其具有明显的电流增敏效果。聚苯二胺膜具有优异的选择透过性能,对抗坏血酸、脲酸、醋氨酚等常见干扰物质具有较强的抗干扰能力。由此制备的生物传感器生物物质探测与分析等领域展现出了广阔的应用前景。The detective performances with the polyphenylenediamine-based amperometric biosensors such as hydrogen peroxide biosensor, glucose biosensor and immunosensor are systematically summarized based on the latest literatures. It is pointed out that the abundance of amino/imino groups in polyphenylenediamine and the morphological structure of its film are beneficial to significant enhancement of loading capacity for noble metal nanoparticles. The highly effective anchor for enzymes by the nanoparticles-loaded polyphenylenediamine and the fast electron transportation by the noble metal nanoparticles both contribute to their remarkably enhanced sensitivity in current. Additionally, the polyphenylenediamine films possess excellent permselectivity with a strong anti-interference ability to common interferents such as ascorbic acid, uric acid and acetaminophen. The biosensors based on the polyphenylenediamine loaded with noble metal nanoparticles exhibit a lower detective limit, higher sensitivity, faster response, better stability and longer lifetime. They have shown great potential applications in the detection and determination for biosubstance.

关 键 词：聚苯二胺 贵金属 纳米粒子 生物传感器 电催化活性 综述 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TB383[自动化与计算机技术—控制科学与工程]