甜瓜枯萎病拮抗内生细菌筛选研究  

Selection of Endophytic Bacteria for Suppression of Melon Wilt Caused by Fusarium oxysporum

作　　者：周婧[1] 高增贵[1] 何秀玲[1] 庄敬华[1] 张小飞[1] 吴海云[1] 

机构地区：[1]沈阳农业大学植物保护学院农业部北方农作物病害免疫重点开放实验室,辽宁沈阳110161

出　　处：《北方园艺》2008年第2期225-227,共3页Northern Horticulture

基　　金：辽宁省自然科学基金资助项目(20032087;20062018);辽宁省"十一.五"科技攻关资助项目(2006215004)

摘　　要：从长势健壮的黄瓜、南瓜和甜瓜苗的根部及茎部分离获得294株内生细菌,与枯萎尖孢镰孢菌进行对峙培养,获得具有抑菌活性细菌18株,占总菌株数的6.1%。再利用生长速率法、孢子萌发法对其进行体外生物活性检测,结果表明,H-2、H-4、H-6、N-7、T-16、T-18等6个菌株的发酵滤液对枯萎镰孢菌的拮抗作用较强,但H-1与H-4对种子萌芽有强烈的抑制作用。H-5、H-6和N8等菌株对甜瓜幼苗具有促生作用,对甜瓜枯萎病的防效分别为75.6%、60.1%和66.7%。H-5菌株对甜瓜枯萎病可能具有潜在的生防功能。Two hundreds and nlnety-four strains of endophytic bacteria were isolated from roots and sterns of healthy cucumber, pumpkin and melon seedlings. By duel test of in vitro screening, about 6. 1% of the total strains screened, 18 antagonistic strains against Fusarium oxysporum f. sp. melonis （Leach） Syn were gotten. Among the 18 strains, 6 strains （H-2, H-4, H-6, N-7, T-16, and T-18） had been proved that their fermented solution inhibited significantly Fusarium oxysporum by using in vitro dilution plate technique test and spore germination test, 2 strains （H-1 and H-4） inhibited melon seed germination. The growth-promoting effects on melon seedling, and control effects against melon wilt of the 18 strains were studied. The results showed that control efficacies of 3 strains, H-5, H-6 and N-8, were 75. 6%, 60. 1% and 66. 7% respectively, meanwhile, their growth promoting effect were found also. H-5 was able to be a biocontrol agent to control the melon wilt Fusarium oxysporum.

关 键 词：甜瓜枯萎病 拮抗内生细菌 生物防治 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]