尿中鸦片类滥用药物的膜式固相萃取及GC/MS/SIM测定  

Determination of Opium Drugs in Urine by Disk Solid Phase Extraction and Gas Chromatography-Mass Spectrometry in Selected Ion Monitoring

作　　者：朱彬玲[1] 

机构地区：[1]福建警察学院,福州350007

出　　处：《中国药事》2008年第2期127-130,共4页Chinese Pharmaceutical Affairs

摘　　要：研究尿中哌替啶、美沙酮、可待因、吗啡、O6-乙酰吗啡等5种鸦片类滥用药物的膜式固相萃取（diskSPE）以及GC／MS／SIM定量测定的方法。采用固相萃取柱萃取，以含2%（v／v）氨水的氯仿-正丙醇（4：1）为洗脱液，对尿中的5种鸦片类滥用药物进行提取。经BSTFA衍生化后再用Gc／MS／SIM测定，分析物的检出限为0．001～0．008μg·mL^-1。同一根萃取柱，连续使用7次，没有出现堵塞和污染，回收率及RSD未见下降，用所建立的diskSPE方法对嫌疑尿样进行分析，与液-液萃取的结论一致。该方法适合于法医毒物分析。The disk SPE-GC/MS/SIM method was applied to the study of opium drugs including pethidine, methadone, codeine, morphine and 6-acetylmorphine in urine. The SPE was performed with disk SPE cartridge, chloroform containing 2% （v/v） ammonia-propyl alcohol （4 ： 1） as the eluent. After derivatization, the detection limits of analytes was 0. 001 -0. 008μg·mL^-1. The extraction column still showed good stability and high reproducibility after continuously used for 7 times. The determination results of cases urine samples were the same by both disk SPE method and LLE method. This method is suitable for forensic toxicological analysis.

关 键 词：膜式固相萃取 鸦片类 GC/MS/SIM 尿 法医毒物分析 

分 类 号：R917[医药卫生—药物分析学]