RP-HPLC法同时测定紫草中紫草素、异丁酰紫草素和β，β-二甲基丙烯酰紫草素的含量  

RP-HPLC determination of shikonin,isobutylshikonin and β,β-dimethylacrylshikonin in Lithospermum erythrorhizon Sieb.et Zucc.

作　　者：韩洁[1] 朱利民[1] 翁新楚[2] 毕开顺[3] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620 [2]上海大学生命科学学院,上海200444 [3]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2008年第1期6-8,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定紫草中紫草素、异丁酰紫草素和β,β-二甲基丙烯酰紫草素含量的方法。方法:采用 RP-HPLC法,Zorbax Eclipse XDB-C_(18)柱(250 mm×4.6 mm,5μm);流动相为含0.5%冰醋酸及0.3%三乙胺的乙腈溶液(A)和含0.5%冰醋酸及0.3%三乙胺的水溶液(B),梯度洗脱,洗脱程序为:0 min(67%A),5 min(70%A),15 min(75%A),30 min(75%A);流速1.0 mL·min^(-1);检测波长516 nm,柱温30 ℃。结果:紫草素、异丁酰紫草素和β,β-二甲基丙烯酰紫草素浓度分别在1.2～12.2μg·mL^(-1)(r=0.9999)、2.5～24.8μg·mL^(-1)(r=0.9998)、11.3～112.8μg·mL^(-1)(r=0.9998)范围内与峰面积呈良好线性关系(n=5)。方法回收率(n=9)分别为100.3%,98.6%,98.4%。结论:该方法简便、快速、准确,重复性好,可用于紫草中紫草素、异丁酰紫草素和β,β-二甲基丙烯酰紫草素含量的同时测定。Objective： To establish a method for the determination of shikonin, isobutylshikonin and β,β - dimethyl- acrylshikonin in Lithospermum erythrorhizon simultaneously. Methods： The RP - HPLC system consisting of a Zorbax Eclipse XDB -C18 colunm（250 mm×4. 6 mm,5 μm）and a mixture of acetonitrile solution（containing 0. 5% glacial acetic acid and 0. 3% triethylamine） （A） and water solution（ containing 0. 5% glacial acetic acid and 0. 3% triethylamine） （B）as the mobile phase in gradient mode was adopted. The concentrations of solvent A were 67% ,70%, 75%, and 75% at 0,5,15, and 30 min,respectively;the detection wavelength was 516 nm;the flow rate was set at 1.0 mL· min^- 1 and the column temperature was 30 ℃. Results ：The linear response ranges of shikonin,isobutylshikonin and β,β - dimethylacrylshikonin were 1.2 - 12. 2 μg· mL^-1 （ r = 0. 9999 ） ,2. 5 - 24. 8μg· mL^-1 （ r = 0. 9998 ） and 11.3 - 112. 8 μg · mL^-1 （ r = 0. 9998 ）, respectively. The average recoveries （ n = 9 ） were 100. 3%, 98. 6% and 98.4% ,respectively. Conclusion：The method is quick, simple, and reproducible for the determination of shikonin, isobutylshikonin and β ,β -dimethylacrylshikonin in Lithospermum erythrorhizon simultaneously.

关 键 词：紫草 紫草素 异丁酰紫草素 β  β-二甲基丙烯酰紫草素 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]