高效液相色谱-荧光法测定人血浆中非索非那定  

HPLC-FLD determination of fexofenadine in human plasma

作　　者：徐海燕[1] 张鹏[1] 王学娅[2] 高靥[1] 申涛[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]辽宁中医药大学职业技术学院,沈阳110101

出　　处：《药物分析杂志》2008年第1期61-64,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定人血浆中非索非那定的高效液相色谱-荧光法。方法:血浆样品经液-液萃取后,以乙腈-水-磷酸-三乙胺(32∶68∶0.8∶0.1)为流动相,流速1.2 mL·min^(-1),采用 Diamonsil C_(18)柱(4.6 mm×150 mm,5μm)分离,以荧光检测器进行检测,激发波长为230 nm,发射波长为290 nm。结果:标准曲线线性范围为0.01～1.0μg·mL^(-1),定量下限为0.01μg·mL^(-1),日内和日间精密度(RSD)均小于9.6%,准确度(RE)在±2.8%以内。结论:该法操作简便、快速、灵敏,适用于非索非那定的临床药动学研究。Objective：To develop an HPLC - FLD method for the determination of fexofenadine in human plasma. Method：After a simple liquid -liquid extraction,fexofenadine and the internal standard were separated on Diamonsil C18 column（4. 6 mm ×150 mm,5 μm） and detected by fluorescence detector. The excitation wavelength was set at 230 nm and the emission wavelength was 290 nm. The mobile phase consisted of acetonitrile - water - phosphoric acid - triethylamine （ 32： 68： 0. 8： 0. 1 ） at a flow rate of 1.2 mL·min ^-1. Results： The linear calibration curve was obtained in the concentration range of 0. 01 - 1.0 μg· mL^-1. The lowest limit of quantification was 0. 01 μg· mL^-1. The intra - day RSD and inter - day RSD were less than 9.6% , and the accuracy （ RE ） was within± 2. 8% calculated from QC samples. Conclusion：The method is proved to be suitable for the clinical investigation of fexofenadine pharmacokinetics, which offers the advantage of simplicity, speed and sensitivity.

关 键 词：非索非那定 高效液相色谱-荧光法 药动学 

分 类 号：R917[医药卫生—药物分析学]