HPLC法测定鼻炎灵滴鼻液中盐酸麻黄碱和马来酸氯苯那敏的含量  

HPLC determination of chlorpheniramine maleate and ephedrine hydrochloride in Biyanling nasal drops

作　　者：何家田[1] 赵兴红[1] 陈瑶[1] 李芳[2] 

机构地区：[1]兰州军区联勤部药品仪器检验所,兰州730050 [2]兰州大学第二医院,兰州730030

出　　处：《药物分析杂志》2008年第1期143-145,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用高效液相色谱法,对鼻炎灵滴鼻液中盐酸麻黄碱、马来酸氯苯那敏进行分离和含量测定。方法:采用 ZORB—AX Extend C_(18)柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.05 mol·L^(-1)磷酸二氢钾溶液(含2%三乙胺,磷酸调 pH 至3.0)(40:60),流速:1.0 mL·min^(-1),检测波长257 nm,柱温:30℃。结果:盐酸麻黄碱和马来酸氯苯那敏浓度分别在0.210～2.096 mg·mL^(-1)(r=0.9998)和79.6～796.0μg·mL^(-1)(r=0.9999)范围内呈良好线性关系;平均回收率分别为96.2%～100.3%和97.7%～101.3%。结论:本法快速、准确、简便。Objective：To establish an HPLC method for separating and determining ephedrine hydrochloride and chlorpheniramine maleate in Biyanling nasal drops simultaneously. Methods ：The ZORBAX Extend C lscolumn（ 150 mm × 4. 6 mm ,μm） was used, mobile phase consisted of methanol- 0. 05 mol · L^-1 potassium dihydrogen phosphate solution（containing 2% triethylamine, adjusted to pH 3.0 with phosphoric acid）（40： 60） at a flow rate of 1.0 mL · min ^- 1, detection wavelength being 257 nm and column temperature being 30 ℃. Results： The standard curves of ephedrine hydrochloride and chlorpheniramine maleate showed good linearity over the range of 0. 210 - 2. 096 mg · mL^- 1 （ r = 0. 9998 ）, 79. 6 - 796. 0 μg · mL^- 1 （ r = 0. 9999 ）, respectively. The average recoveries of the method were 96. 2% - 100. 3% and 97.7% - 101.3% ,respectively. Conclusion：The method is simple,rapid, sensitive and reproducible.

关 键 词：盐酸麻黄碱 马来酸氯苯那敏 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]