大气颗粒物不同组分对大鼠肝细胞DNA合成的影响  

The Effect of Subfractionated Groups of Airborne Particles on DNA Synthesis in Primary Rat Liver Cells

作　　者：赵贤四[1,2] 朱惠刚[1,2] 

机构地区：[1]上海铁道大学医学院预防医学教研室 [2]上海医科大学

出　　处：《环境与健康杂志》1997年第4期149-152,共4页Journal of Environment and Health

基　　金：上海市环保局85重点资助

摘　　要：为探讨大气颗粒物不同组分对大鼠肝细胞ＤＮＡ合成的影响，采用大鼠肝细胞的程序外ＤＮＡ合成（ＵＤＳ）试验研究上海市大气颗粒物不同有机组分对大鼠肝细胞ＤＮＡ的损伤作用，以确定不同性质的有机成分的遗传毒性。结果表明：各组分均具有不同程度的ＤＮＡ损伤作用，以多环芳烃组最明显，夏季样品结果变异较大。说明上海市大气颗粒物中遗传毒性物质的种类较多，除多环芳烃外还有许多其它成分值得重视。In this study,rat liver unscheduled DNA synthesis(UDS) assay was used to detect the DNA damage induced by the five subfractionated groups of organic extract of airborne particles in Shanghai,so as to identify the genetic toxicity of each group.The results showed that all of the five groups could induce some UDS responses in primary rat liver cells.The group of PAHs had the strongest DNA damage ability.Meanwhile,the more variation of UDS level were observed in the results of summer samples.It suggested that many genotoxic substances,besides PAHs,presented in airborne particles which should be further investigated.

关 键 词：大气 颗粒物 DNA 合成 修复 大鼠 肝细胞 

分 类 号：X513.032.2[环境科学与工程—环境工程]