浑球红细菌谷氨酸合酶大亚单位基因(gltB)的序列分析  

NUCLEOTIDE SEQUENCE OF gltB GENE ENCODING THE LARGE SUBUNIT OF RHODOBACTER SPHAEROIDES GLUTAMATE SYNTHASE

作　　者：鲁涛[1] 吴永强[1] 宋鸿遇[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《微生物学报》1997年第2期87-94,共8页Acta Microbiologica Sinica

基　　金：国家高技术发展(863)计划;国家自然科学基金资助项目。

摘　　要：测定了浑球红细菌(Rhodobacter sphaeroides)谷氨酸合酶大亚单位基因(gltB)及其5'端和3'端的序列,全长为5510bp。序列分析表明,R. sphaeroides gltB基因全长为4636bp。从核苷酸序列推测其蛋白质分子量约为164kD。R. sphaeroides gltB基因与Azospirillum brasilense和Escherichia coli的gltB基因DNA序列有很高的同源性。其蛋白质氨基酸序列与A. brasilense gltB基因产物GltB也具有很高的同源性。此外,还对R. sphaeroides GltB的各可能功能区进行了分析,发现它们具有很高的保守性。The complete nucleotide sequence of a 5.4-kb chromosomal EcoRI-Sall fragment was determined, which contains the structural gene (gltE) for the large subunit of Rhodobacter sphaeroides glutamate synthase, as well as the 5' - and 3'-flanking regions. A open reading frame of 4636 base pairs was identified as R. sphaeroides gltB gene- The MW of the large subunit, as deduced from the nucleotide sequence, was estimated as 164 kD. Comparision of the nucleotide sequences revealed a high similarity among gltB genes of R. sphaeroides, Azospirillum brasilense and Escherichia coll The deduced amino acid sequence of R. sphaeroides GltB showed a high similarity with that of A. brasilense GltB.

关 键 词：浑球红细菌 谷氨酸合酶基因 DNA序列 

分 类 号：Q939.103[生物学—微生物学]