Sindbis病毒的繁殖与宿主细胞BHK—21的凋亡  

THE MUTIPLICATION OF SINDBIS VIRUS AND HOST BHK-21 CELL APOPTOSIS

作　　者：张小青[1] 裘霁[1] 丁明孝[1] 任显辉[2] 邱殷庆[2] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]香港中文大学解剖学系

出　　处：《微生物学报》1997年第3期165-170,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助

摘　　要：详细报道了Sindbis病毒诱导BHK-21细胞凋亡的过程,病毒感染6h后即可观测到核染色质的断裂,病毒感染12h后染色质可见明显的凝集,感染后24h DNA电泳出现明显的DNA“阶梯”(DNA ladder)。电镜观察更清楚地显示了凋亡小体形成的某些细节:在染色质凝集处核外膜突起,最后与细胞核分离形成凋亡小体。在此基础上将一段病毒非结构蛋白nsP2基因克隆到真核表达载体pMAMneo中,并得到瞬间表达,在其中一些细胞中出现DNA断裂这一细胞凋亡的基本特征,通过对nsP2氨基酸序列的分析,结合以前的实验结果推测nsP2可能与诱导细胞凋亡直接相关。The process of apoptosis of BHK-21 cells induced by Sindbis virus (SbV) infection is reported here in details. The nuclear DNA cleavage can be first detected at 6h after SbV infection, followed by the chromatin margination and condensation at 12h and the DNA ladder can be detected at 24h after infection. The details of apoptosis body s formation can be revealed by electron microscopy: First, the outer nuclear membrane protrudes where the condensed chromatin accumulates, then the chromatin get into the budding area with the inner nuclear membrane and separate from the nuclear. Progressively, we cloned the gene of SbV nonstructure protein 2 (nsP2) into the eukaryotic expression vector pMAMneo, and it can be expressed transiently. The DNA cleavages, the basic characteristics of apoptosis also can be detected in some cells. Together with the sequence analysis of nsP2 and the other former results, we conclude that the nsP2 may have direct relation with the SbV-induced apoptosis.

关 键 词：病毒 细胞凋亡 Sindbis病毒 繁殖 宿主细胞 

分 类 号：Q939.43[生物学—微生物学]