多能硫杆菌RubisCO基因同源性分析  

HOMOLOGY ANALYSIS OF RUBISCO GENE OF THIOBACILLUS VERSUTUS WITH EXTREMELLY ACIDOPHILIC THIOBACILLI

作　　者：刘振盈[1] 颜望明[1] 徐海岩 郑雷 罗小平 

机构地区：[1]山东大学生命科学院微生物技术国家重点实验室,济南250100

出　　处：《微生物学报》1997年第3期179-183,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助课题;山东省自然科学基金资助课题

摘　　要：以氧化亚铁硫杆菌1,5—二磷酸核酮糖羧化酶/加氧酶(RubisCO)基因为探针,与氧化硫硫杆菌和多能硫杆菌的染色体DNA杂交。结果表明,氧化硫硫杆菌的染色体DNA能够与氧化亚铁硫杆菌RubisCO基因探针杂交。而多能硫杆菌不能与其杂交,然而却能够与球形红杆菌RubisCO基因探针杂交,同源性高。由于RubisCO在进化上的高度保守性,因此认为它们在RubisCO进化关系上应属于不同的类群。The chromosomal DNA of Thiobacillus thiooxidans and T. versutus was digestedwith restriction enzymes, blotted to nylon membrane by the way of Southern, and hybridized with the gene probe of ribulose 1, 5 - bisphosphate carboxylase /oxygenase (RubisCO) from T. ferrooxidans. The result showed that T. thiooxidans exhibited high homology with the probe anc T. versutus was less homology with it. However T. versutus can hybridize with the gene probe of RubisCO from Rhodobacter sphaeroides, which indicated that they had high homology with each other. As RubisCO was highly reserved in the evolutionary, they should be divided into different group.

关 键 词：硫杆菌属 二磷酸核酮糖 羧化酶 加氧酶 基因分析 

分 类 号：Q939.103[生物学—微生物学]