一种新的人兽共患传染病——狐狸阴道加德纳氏菌病的研究 Ⅵ.阴道加德纳氏菌病灭活疫苗的研制  

STUDY ON THE INACTIVATED VACCINE OF GARDNERELLA VAGINALIS OF FOX VI. EXPERIMENTAL DEVELOPMENT OF THE INACTIVATED VACCINE OF GARDNERELLA VAGINALIS OF FOX

作　　者：严忠诚[1] 闫新华[1] 闫喜军[1] 栾凤英 王长凤[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《微生物学报》1997年第3期217-227,共11页Acta Microbiologica Sinica

基　　金：农业部畜牧业"八五"重点研究专题

摘　　要：选用免疫原性优良的我国狐狸阴道加德纳氏菌主要流行型血清Ⅰ型GVF44号菌株,先后试验研制了氢氧化铝胶、蜂胶和油佐剂灭活苗。试验结果表明,铝胶苗的安全性优,免疫剂量为40亿菌/1ml,3倍免疫剂量的铝胶灭活苗接种狐狸后无任何不良反应,免疫后21d经100个ID_(50)强毒的攻击,对Ⅰ型的保护率达92％。对Ⅱ、Ⅲ型的保护率达80％以上。免疫持续期为6个月,4～10℃条件下可保存10个月。通过田间试验应用证实该疫苗安全、有效。The aluminum hydroxide gel, propolis and oil adjuvant inactivated vaccines were developed by silecting good immunity, representing principal epidemic serotype I GVF44 of Gardnerella vaginalis of fox in civil. The results showed that the aluminum hydroxide gel vaccine in safety was better than propolis and oil adjuvant vaccines, the most optimal inactivated concentration of formalin is 0.1%. Efficient immunity doses are 4 billon bacteria /1ml. There were on any bad influences to occur for fox after the aluminum hydroxide gel of three times immunity doses were injected. The inoculated foxes could resist challenge of a hundred ID50 virulent strains after the vaccine was injected to foxes for 21 d. Immunity period of the vaccine is 6 months, storage period is 10 months at 4- 10℃. The field experiments confirmed that the vaccine possessed accuracy, stability and reliability.

关 键 词：狐狸 阴道加德纳氏菌 阴道疾病 灭活疫苗 研制 

分 类 号：R711.73[医药卫生—妇产科学] R392－33[医药卫生—临床医学]