金藻基因组DNA的提取与PCR扩增  

Genomic DNA extraction and PCR amplication of haptophytes

作　　者：杨泽民[1] 章群[2] 谢数涛[2] 吴任[2] 

机构地区：[1]广东药学院基础学院 [2]暨南大学水生生物研究所

出　　处：《生物学杂志》2008年第1期60-63,共4页Journal of Biology

摘　　要：以金藻门群体和单细胞藻类的典型代表——球形棕囊藻和绿色巴夫藻为研究对象,对金藻基因组DNA的提取方法进行了深入研究,并以psbA基因的PCR扩增结果对4种DNA提取方法进行了检测。结果显示,PVP法和高盐法安全、快速、经济,获得的基因组DNA产量高,但DNA杂质含量也较高。CTAB法虽然也具有安全、快速等优势,但提取的DNA产量较低。玻璃粉法提取的DNA质量好,产量也较高,但操作略为烦琐,经济费用高。结论:就球形棕囊藻而言,玻璃粉法是4种方法中最好的DNA提取方法。就绿色巴夫藻而言,4种方法都行,但是从经济和方便考虑,以高盐法为最好。Phaeocystis globosa and Pavlova viridis which are colony and single cell representative were used as samples. Genomic DNA extraction from those haptophytes was studied. 4 methods for DNA extraction were examined, based on PCR amplification of psbA gene. The results showed that both salt-extraction of high concentration and PVP - extraction were safe, quick and economical methods. The high-yield DNA was extracted by the two methods, but impurity. The method of CTAB-extraction was also safe and quick, but the yield of genomic DNA was low. The glass bead-extraction can obtain high-yield and purity genomic DNA, but process is complex and expen- sive. For P. globosa, the method of glass bead-extraction was the best one among 4 methods. For P. viridis, considered economical and convenient factors, the method of salt-extraction of high was the best.

关 键 词：球形棕囊藻 绿色巴夫藻 高盐法 PVP法 玻璃粉法 CTAB法 

分 类 号：Q503[生物学—生物化学]